Cell and Tissue Research

, Volume 356, Issue 2, pp 391–403

Expansion on a matrix deposited by nonchondrogenic urine stem cells strengthens the chondrogenic capacity of repeated-passage bone marrow stromal cells

  • Ming Pei
  • Jingting Li
  • Ying Zhang
  • Guihua Liu
  • Lei Wei
  • Yuanyuan Zhang
Regular Article

Abstract

Human urine-derived stem cells (hUSCs) are a newly found type of stem cell with a potential for therapeutic application in urology. The aim of this study is to investigate whether hUSCs contribute to cartilage regeneration. Despite their characterization with multi-lineage differentiation capacities, in terms of osteogenesis, adipogenesis and myogenesis, hUSCs do not show the ability to differentiate into chondrocytes. Human bone marrow stromal cells (hBMSCs) are a tissue-specific stem cell for endochondral bone formation; however, repeated-passage hBMSCs have a lower capacity for chondrogenic differentiation. We found that the extracellular matrix (ECM) deposited by hUSCs (UECM) can greatly recharge repeated-passage hBMSCs toward chondrogenic differentiation, a result that might be explained by trophic factors released from hUSCs being immobilized in UECM. We also found that ECM from repeated-passage hBMSCs (BECM) have a limited rejuvenation effect. The Wnt11-mediated noncanonical signaling pathway might be responsible for UECM-mediated hBMSC rejuvenation and subsequent chondrogenic differentiation. Our data indicate that commercially available UECM from young healthy donors might represent a simple and promising approach for autologous hBMSC rejuvenation. This study also provides an excellent model for investigating the effect of trophic factors released by stem cells on tissue regeneration without interference by stem cell differentiation.

Keywords

Decellularized stem cell matrix Monolayer expansion Urine-derived stem cells Bone marrow stromal cells Endochondral bone formation Human 

Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Ming Pei
    • 1
    • 2
    • 3
  • Jingting Li
    • 1
    • 2
  • Ying Zhang
    • 1
    • 3
  • Guihua Liu
    • 4
  • Lei Wei
    • 5
  • Yuanyuan Zhang
    • 4
  1. 1.Stem Cell and Tissue Engineering Laboratory, Department of OrthopaedicsWest Virginia UniversityMorgantownUSA
  2. 2.Exercise PhysiologyWest Virginia UniversityMorgantownUSA
  3. 3.Mechanical & Aerospace EngineeringWest Virginia UniversityMorgantownUSA
  4. 4.Wake Forest Institute for Regenerative MedicineWinston-SalemUSA
  5. 5.Molecular Biology Laboratory, Department of OrthopaedicsAlpert Medical School of Brown UniversityProvidenceUSA

Personalised recommendations