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Expression of caveolin-1 and podocalyxin in rat lungs challenged with 2-kDa macrophage-activating lipopeptide and Flt3L

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Abstract

Caveolin-1 is one of the important regulators of vascular permeability in inflamed lungs. Podocalyxin is a CD34 protein expressed on vascular endothelium and has a role in podocyte development in the kidney. Few data are available on the expression of caveolin-1 and podocalyxin in lungs challenged with Toll-like receptor 2 (TLR2) agonists such as mycoplasma-derived macrophage activating lipopeptide or with immune modulators such as Fms-like tyrosine kinase receptor-3 ligand (Flt3L), which expands dendritic cell populations in the lung. Because of the significance of pathogen-derived molecules that act through TLR2 and of the role of immune modulators in lung physiology, we examine the immunohistochemical expression of caveolin-1 and podocalyxin in lungs from rats challenged with a 2-kDa macrophage-activating lipopeptide (MALP-2) and Flt3L. Normal rat lungs expressed caveolin-1 in alveolar septa, vascular endothelium and airway epithelium, especially along the lateral borders of epithelial cells but not in alveolar macrophages. MALP-2 and Flt3L decreased and increased, respectively, the expression of caveolin-1. Caveolin-1 expression seemed to increase in microvessels in bronchiole-associated lymphoid tissue (BALT) in Flt3L-challenged lungs but not in normal or MALP-2-treated lungs. Podocalyxin was absent in the epithelium and alveolar macrophages but was present in the vasculature of control, Flt3L- and MALP-2-treated rats. Compared with control and MALP-2-treated rats, Flt3L-treated lungs showed greater expression of podocalyxin in BALT vasculature and at the interface of monocytes and the endothelium. These immunohistochemical data describing the altered expression of caveolin-1 and podocalyxin in lungs treated with MALP-2 or Flt3L encourage further mechanistic studies on the role of podocalyxin and caveolin-1 in lung inflammation.

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Authors and Affiliations

  1. Institute of Anatomy and Cell Biology, Saarland University, Homburg, Saar, Germany

    T. Tschernig

  2. Institute of Immunomorphology, Medical School of Hannover, Hannover, Germany

    R. Pabst

  3. Institute of Anatomy, Medical Faculty, University of Technology Dresden, Dresden, Germany

    M. Kasper

  4. Department of Veterinary Biomedical Sciences, University of Saskatchewan, 52 Campus Drive, Saskatoon, SK, S7N 5B4, Canada

    Mustafa El-Hadi & B. Singh

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  1. T. Tschernig
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  2. R. Pabst
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  4. Mustafa El-Hadi
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  5. B. Singh
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Correspondence to B. Singh.

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The work reported in this study was supported by grants from the Natural Sciences and Engineering Research Council of Canada. Dr. Mustafa al-Hadi was a recipient of an NSERC-Undergraduate Student Research Award.

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Tschernig, T., Pabst, R., Kasper, M. et al. Expression of caveolin-1 and podocalyxin in rat lungs challenged with 2-kDa macrophage-activating lipopeptide and Flt3L. Cell Tissue Res 356, 207–216 (2014). https://doi.org/10.1007/s00441-013-1771-y

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  • DOI: https://doi.org/10.1007/s00441-013-1771-y

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