Cell and Tissue Research

, Volume 354, Issue 2, pp 507–519

Immunolocalization and distribution of functional temperature-sensitive TRP channels in salivary glands

  • Ubaidus Sobhan
  • Masaki Sato
  • Takashi Shinomiya
  • Migiwa Okubo
  • Maki Tsumura
  • Takashi Muramatsu
  • Mitsuru Kawaguchi
  • Masakazu Tazaki
  • Yoshiyuki Shibukawa
Regular Article

DOI: 10.1007/s00441-013-1691-x

Cite this article as:
Sobhan, U., Sato, M., Shinomiya, T. et al. Cell Tissue Res (2013) 354: 507. doi:10.1007/s00441-013-1691-x

Abstract

Transient receptor potential (TRP) cation channels are unique cellular sensors involved in multiple cellular functions. Their role in salivary secretion remains to be elucidated. The expression and localization of temperature-sensitive TRP channels in salivary (submandibular, sublingual and parotid) glands were analyzed by immunohistochemistry and quantitative real-time reverse transcription plus the polymerase chain reaction (RT-PCR). The effects of various TRP channel agonists on carbachol (CCh)-induced salivary secretion in the submandibular gland and on the intracellular Ca2+ concentration ([Ca2+]i) in a submandibular epithelial cell line were also investigated. Immunohistochemistry revealed the expression of TRP-melastatin subfamily member 8 (TRPM8) and TRP-ankyrin subfamily member 1 (TRPA1) in myoepithelial, acinar and ductal cells in the sublingual, submandibular and parotid glands. In addition, TRP-vanilloid subfamily member 1 (TRPV1), TRPV3 and TRPV4 were also expressed in myoepithelial, acinar and ductal cells in all three types of gland. Quantitative real-time RT-PCR results demonstrated the mRNA expression of TRPV1, TRPV3, TRPV4, TRPM8 and TRPA1 in acinar and ductal cells in these salivary glands. Perfusion of the entire submandibular gland with the TRPV1 agonist capsaicin (1 μM) via the submandibular artery significantly increased CCh-induced salivation, whereas perfusion with TRPM8 and TRPA1 agonists (0.5 μM WS12 and 100 μM allyl isothiocyanate) decreased it. Application of agonists for each of the thermosensitive TRP channels increased [Ca2+]i in a submandibular epithelial cell line. These results indicate that temperature-sensitive TRP channels are localized and distributed in acinar, ductal and myoepithelial cells in salivary glands and that they play a functional role in the regulation and/or modulation of salivary secretion.

Keywords

TRP channels Parotid gland Submandibular gland Sublingual gland Salivary secretion Rat 

Supplementary material

441_2013_1691_MOESM1_ESM.docx (30 kb)
Table 1(DOCX 29 kb)

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Ubaidus Sobhan
    • 1
  • Masaki Sato
    • 1
  • Takashi Shinomiya
    • 1
    • 2
  • Migiwa Okubo
    • 1
    • 2
  • Maki Tsumura
    • 1
    • 3
  • Takashi Muramatsu
    • 1
    • 4
  • Mitsuru Kawaguchi
    • 2
  • Masakazu Tazaki
    • 3
  • Yoshiyuki Shibukawa
    • 1
    • 3
  1. 1.Oral Health Science Center hrc8Tokyo Dental CollegeChibaJapan
  2. 2.Department of PharmacologyTokyo Dental CollegeChibaJapan
  3. 3.Department of PhysiologyTokyo Dental CollegeChiba-cityJapan
  4. 4.Department of Endodontics and Clinical CariologyTokyo Dental CollegeChibaJapan

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