Cell and Tissue Research

, Volume 353, Issue 1, pp 41–52

Comparative evaluation of in vivo osteogenic differentiation of fetal and adult mesenchymal stem cell in rat critical-sized femoral defect model

  • Chris Hyunchul Jo
  • Pil Whan Yoon
  • Hyang Kim
  • Kyung Sun Kang
  • Kang Sup Yoon
Regular Article

Abstract

Mesenchymal stem cells (MSCs) can be obtained from various sources. MSCs from different origins appear to have different preferences for differentiation. In this study, we have compared the in vivo osteogenic potential of adult MSCs from adipose tissue (AT) and bone marrow (BM) with fetal MSCs from umbilical cord (UC) and umbilical cord blood (UCB) by using a rat critical-sized femoral defect model. We have also sought to determine whether pretreatment with an osteogenic medium promotes osteogenesis in MSCs. Study groups were divided as follows: (1) defect only, (2) scaffold only, (3) AT MSCs in scaffolds, (4) BM MSCs in scaffolds, (5) UC MSCs in scaffolds and (6) UCB MSCs in scaffolds. Groups with MSCs were further divided with respect to their pretreatment. At 12 weeks after surgery, in vivo osteogenesis was measured radiographically and by micro-computed tomography (CT). Based on quantitative assessment by micro-CT, no significant difference of the mean bone volume fraction value (BV/TV) was seen between adult MSCs (AT and BM MSCs) and fetal MSCs (UC and UCB MSCs). The mean BV/TVs were significantly higher in non-pretreated BM MSC (14.2±1.4%) and UCB MSC (14.0±1.2%) and pretreated UC MSC (14.8±2.0%) than in those with the scaffold only (11.3±1.3%; P<0.05). In addition, AT (from 10.4±1.2% to 13.1±2.2%) and UC (from 10.3±0.7% to 14.8±2.0%) MSCs from solid tissues showed a significant increase in the mean BV/TV with pretreatment (P<0.05). In contrast, BM MSC (from 14.2±1.4% to 10.9±1.2%) and UCB MSC (from 14.0±1.2% to 11.6±1.0%) from non-solid tissues showed a significant decrease with pretreatment (P<0.05).

Keywords

In vivo osteogenesis Fetal mesenchymal stem cells Adult mesenchymal stem cells Pretreatment Critical-sized femoral defect model Rat Human 

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Chris Hyunchul Jo
    • 1
  • Pil Whan Yoon
    • 1
  • Hyang Kim
    • 2
  • Kyung Sun Kang
    • 3
  • Kang Sup Yoon
    • 4
  1. 1.Department of Orthopedic Surgery, Seoul National University Boramae HospitalSeoul National University College of MedicineSeoulSouth Korea
  2. 2.Stem Cell & Orthopedic Research Laboratory, Department of Orthopedic SurgerySeoul National University Boramae HospitalSeoulSouth Korea
  3. 3.Adult Stem Cell Research Center & Laboratory of Stem Cell and Tumor Biology, College of Veterinary MedicineSeoul National UniversitySeoulSouth Korea
  4. 4.Department of Orthopedic Surgery & Cell Therapy Center, Seoul National University Boramae HospitalSeoul National University College of MedicineSeoulSouth Korea

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