Septin9 is involved in T-cell development and CD8+ T-cell homeostasis
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SEPTIN9 (SEPT9) is a filament-forming protein involved in numerous cellular processes. We have used a conditional knock out allele of Sept9 to specifically delete Sept9 in T-cells. As shown by fluorescence-activated cell sorting, loss of Sept9 at an early thymocyte stage in the thymus results in increased numbers of double-negative cells indicating that SEPT9 is involved in the transition from the double-negative stage during T-cell development. Accordingly, the relative numbers of mature T-cells in the periphery are decreased in mice with a T-cell-specific deletion of Sept9. Proliferation of Sept9-deleted CD8+ T-cells from the spleen is decreased upon stimulation in culture. The altered T-cell homeostasis caused by the loss of Sept9 results in an increase of CD8+ central memory T-cells.
KeywordsSept9 T-cell development CD8+ T-cells T-cell homeostasis T-cell proliferation Mice (Sept9cond:Mx-Cre)
The authors thank Lone Højgaard Nielsen for technical assistance, and Charlotte Christie Petersen, Rodrigo Labouriau and Bo Porse for advice regarding the set-up and analysis of experiments. Flow cytometry/cell sorting was performed at the FACS Core Facility, The Faculty of Health Sciences, Aarhus University, Denmark.
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