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Cell and Tissue Research

, Volume 350, Issue 1, pp 13–26 | Cite as

Tubulin-binding cofactor B is a direct interaction partner of the dynactin subunit p150Glued

  • Georges F. Kuh
  • Marianne Stockmann
  • Marie Meyer-Ohlendorf
  • Leonhard Linta
  • Christian Proepper
  • Albert C. Ludolph
  • Juergen Bockmann
  • Tobias M. BoeckersEmail author
  • Stefan LiebauEmail author
Regular Article

Abstract

The dynactin p150Glued subunit, encoded by the gene DCTN1, is part of the dynein-dynactin motor protein complex responsible for retrograde axonal transport in motor neurons. The p150 subunit is a candidate gene for neurodegenerative diseases, in particular motor neuron and extrapyramidal diseases. Tubulin-binding cofactors are believed to be involved in tubulin biogenesis and degradation and therefore to contribute to microtubule functional diversity and regulation. A yeast-two-hybrid screen for putative interacting proteins of dynactin p150Glued has revealed tubulin-folding cofactor B (TBCB). We analyzed the interaction of these proteins and investigated the impact of this complex on the microtubule network in cell lines and primary hippocampal neurons in vitro. We especially concentrated on neuronal morphology and synaptogenesis. Overexpression of both proteins or depletion of TBCB alone does not alter the microtubule network and/or neuronal morphology. The demonstration of the interaction of the transport molecule dynactin and the tubulin-regulating factor TBCB is thought to have an impact on several cellular mechanisms. TBCB expression levels have been found to have only a subtle influence on the microtubule network and neuronal morphology. However, overexpression of TBCB leads to the decreased localization of p150 to the microtubule network that might result in a functional modulation of this protein complex.

Keywords

Tubulin-binding cofactor Dynactin p150 Microtubules Neuronal morphology Synaptogenesis Cell culture 

Notes

Acknowledgments

The authors thank Sabine Seltenheim and Renate Zienecker for excellent technical support.

Supplementary material

441_2012_1463_MOESM1_ESM.pdf (2.4 mb)
ESM 1 (PDF 2480 kb)

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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Georges F. Kuh
    • 1
  • Marianne Stockmann
    • 1
  • Marie Meyer-Ohlendorf
    • 1
  • Leonhard Linta
    • 1
  • Christian Proepper
    • 1
  • Albert C. Ludolph
    • 2
  • Juergen Bockmann
    • 1
  • Tobias M. Boeckers
    • 1
    Email author
  • Stefan Liebau
    • 1
    Email author
  1. 1.Institute for Anatomy & Cell BiologyUlm UniversityUlmGermany
  2. 2.Department of NeurologyUlm UniversityUlmGermany

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