Recellularization of decellularized human adipose-tissue-derived extracellular matrix sheets with other human cell types
- 1.2k Downloads
Decellularized human extracellular matrices (ECMs) are an extremely appealing biomaterial for tissue engineering and regenerative medicine. In this study, we decellularized human adipose tissue, fabricated a thin ECM sheet and explored the potential of this human adipose-derived ECM sheet as a substrate to support the formation of tissues other than adipose tissue. Acellular ECM sheets were fabricated from human adipose tissue through successive physical and chemical treatments: homogenization, centrifugation, casting, freeze-drying and sodium dodecyl sulfate treatment. The ECM sheets exhibited good mechanical properties, despite their porous structure. They degraded quickly in the presence of collagenase and the degradation rate increased with the collagenase concentration in phosphate-buffered saline. Five different human cell types, covering a broad range of cells and applications (normal human dermal fibroblasts, human aortic smooth muscle cells, human chondrocytes, human umbilical vein endothelial cells and human adipose-derived stem cells), were seeded onto the ECM sheets. All the human cell types spread well, proliferated and were successfully integrated into the decellularized ECM sheet. Overall, the results suggest that recellularized ECM sheets are a promising substitute for defective or damaged human tissues.
KeywordsDecellularization Recellularization Extracellular matrix Adipose tissue Tissue engineering
This work was supported by the Basic Science Research Program (grant no. 2009-0075546) and the Bio & Medical Technology Development Program (grant no. 2011-0019774) of the National Research Foundation of Korea (NRF) funded by the Korean government (MEST). Our research (grant no. 00046001) was also supported by Business for Academic-Industrial Cooperative Establishments funded by the Korea Small and Medium Business Administration in 2011.
- Schopka S, Schmid FX, Hirt S, Birnbaum DE, Schmid C, Lehle K (2009) Recellularization of biological heart valves with human vascular cells: in vitro hemocompatibility assessment. J Biomed Mater Res B Appl Biomater 88:130–138Google Scholar
- Sodian R, Hoerstrup SP, Sperling JS, Daebritz SH, Martin DP, Schoen FJ, Vacanti JP, Mayer JE Jr (2000) Tissue engineering of heart valves: in vitro experiences. Ann Thorac Surg 70:140–144Google Scholar
- van Tienen TG, Heijkants RG, Buma P, de Groot JH, Pennings AJ, Veth RP (2002) Tissue ingrowth and degradation of two biodegradable porous polymers with different porosities and pore sizes. Biomaterials 23:1731–1738Google Scholar
- Wake MC, Patrick CW Jr, Mikos AG (1994) Pore morphology effects on the fibrovascular tissue growth in porous polymer substrates. Cell Transplant 3:339–343Google Scholar