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Cell and Tissue Research

, Volume 348, Issue 3, pp 559–567 | Cite as

Recellularization of decellularized human adipose-tissue-derived extracellular matrix sheets with other human cell types

  • Beob Soo Kim
  • Ji Suk Choi
  • Jae Dong Kim
  • Young Chan Choi
  • Yong Woo ChoEmail author
Regular Article

Abstract

Decellularized human extracellular matrices (ECMs) are an extremely appealing biomaterial for tissue engineering and regenerative medicine. In this study, we decellularized human adipose tissue, fabricated a thin ECM sheet and explored the potential of this human adipose-derived ECM sheet as a substrate to support the formation of tissues other than adipose tissue. Acellular ECM sheets were fabricated from human adipose tissue through successive physical and chemical treatments: homogenization, centrifugation, casting, freeze-drying and sodium dodecyl sulfate treatment. The ECM sheets exhibited good mechanical properties, despite their porous structure. They degraded quickly in the presence of collagenase and the degradation rate increased with the collagenase concentration in phosphate-buffered saline. Five different human cell types, covering a broad range of cells and applications (normal human dermal fibroblasts, human aortic smooth muscle cells, human chondrocytes, human umbilical vein endothelial cells and human adipose-derived stem cells), were seeded onto the ECM sheets. All the human cell types spread well, proliferated and were successfully integrated into the decellularized ECM sheet. Overall, the results suggest that recellularized ECM sheets are a promising substitute for defective or damaged human tissues.

Keywords

Decellularization Recellularization Extracellular matrix Adipose tissue Tissue engineering 

Notes

Acknowledgements

This work was supported by the Basic Science Research Program (grant no. 2009-0075546) and the Bio & Medical Technology Development Program (grant no. 2011-0019774) of the National Research Foundation of Korea (NRF) funded by the Korean government (MEST). Our research (grant no. 00046001) was also supported by Business for Academic-Industrial Cooperative Establishments funded by the Korea Small and Medium Business Administration in 2011.

Supplementary material

441_2012_1391_MOESM1_ESM.doc (1.4 mb)
ESM 1 (DOC 1.36 mb)

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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Beob Soo Kim
    • 1
  • Ji Suk Choi
    • 1
  • Jae Dong Kim
    • 1
  • Young Chan Choi
    • 1
  • Yong Woo Cho
    • 1
    Email author
  1. 1.Department of Chemical Engineering and Department of BionanotechnologyHanyang UniversityAnsanRepublic of Korea

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