Cell and Tissue Research

, Volume 348, Issue 1, pp 141–153 | Cite as

Morphological and functional characterization of a novel Na+/K+-ATPase-immunoreactive, follicle-like structure on the gill septum of Japanese banded houndshark, Triakis scyllium

  • Souichirou TakabeEmail author
  • Keitaro Teranishi
  • Shin Takaki
  • Makoto Kusakabe
  • Shigehisa Hirose
  • Toyoji Kaneko
  • Susumu Hyodo
Regular Article


In teleost fishes, it is well-established that the gill serves as an important ionoregulatory organ in addition to its primary function of respiratory gas exchange. In elasmobranch fish, however, the ionoregulatory function of the gills is still poorly understood. Although mitochondria-rich (MR) cells have also been found in elasmobranch fish, these cells are considered to function primarily in acid-base regulation. In this study, we found a novel aggregate structure made up of cells with basolaterally-expressed Na+/K+-ATPase (NKA), in addition to NKA-immunoreactive MR cells that have already been described in the gill filament and lamella. The cell aggregates, named follicularly-arranged NKA-rich cells (follicular NRCs), were found exclusively in the epithelial lining of the venous web in the cavernous region of the filament and the inter-filamental space of the gill septum. The follicular NRCs form a single-layered follicular structure with a large lumen leading to the external environment. The follicular NRCs were characterized by: (i) well-developed microvilli on the apical membrane, (ii) less prominent infoldings of the basolateral membrane and (iii) typical junction structures including deep tight junction between cells. In addition, large numbers of vesicles were observed in the cytoplasm and some of them were fused to the lateral membrane. The follicular NRCs expressed Na+/H+ exchanger 3 and Ca2+ transporter 1. The follicular NRCs thus have the characteristics of absorptive ionoregulatory cells and this suggests that the elasmobranch gill probably contributes more importantly to body fluid homeostasis than previously thought.


Elasmobranch Gill Na+/K+-ATPase rich cell Follicular structure Ion regulation 



We sincerely thank Prof. Christopher A Loretz of Univ. of Buffalo for critical reading of the manuscript and Dr. Soichi Watanabe of Univ. of Tokyo for his invaluable discussion and encouragement. This study was supported by Grants-in-Aid for Scientific Research to TK and SH.


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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Souichirou Takabe
    • 1
    • 2
    Email author
  • Keitaro Teranishi
    • 3
  • Shin Takaki
    • 1
  • Makoto Kusakabe
    • 1
  • Shigehisa Hirose
    • 4
  • Toyoji Kaneko
    • 3
  • Susumu Hyodo
    • 1
  1. 1.Laboratory of Physiology, Atmosphere and Ocean Research InstituteUniversity of TokyoTokyoJapan
  2. 2.KashiwaJapan
  3. 3.Department of Aquatic Bioscience, Graduate School of Agricultural and Life SciencesUniversity of TokyoTokyoJapan
  4. 4.Department of Biological SciencesTokyo Institute of TechnologyYokohamaJapan

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