Immunostimulatory activity of ConBr: a focus on splenocyte proliferation and proliferative cytokine secretion
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Lectins constitute a class of glycoproteins, which are capable of selectively and reversibly binding to carbohydrates, distinguishing small structural differences in complex oligosaccharides. Studies have shown that the binding of lectins to cell-surface carbohydrates can lead to various effects such as cellular proliferation, histamine release and cytokine production. Canavalia brasiliensis lectin (ConBr) is a (D-mannose) D-glucose lectin. In this study, murine splenocytes were cultured to determine the effect of ConBr on cell proliferation, nitric oxide (NO) release and cytokine secretion. In addition, cellular viability assays were performed to evaluate any mitogenic activity induced by this lectin. ConBr significantly increased cell proliferation with minimal cell damage. This lectin was able to induce an increased production of cytokines such as IL-2, IL-6 and IFN-γ and a decreased production of IL- 10. The release of NO was also observed. The results of this study indicate that ConBr could potentially be used as an immunomodulator.
KeywordsCanavalia brasiliensis ConBr Lectins Glucose/mannose binding-lectin Proliferative activity
We are grateful to Lucas Ferreira da Rocha and Maria da Conceição Batista for their technical assistance. This work was supported by CAPES (Fundação de Coordenação de Aperfeiçoamento de Pessoal de Nível Superior), FACEPE (Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco, processo APQ-0493-2.11/08), and the Aggeu Magalhães Research Center of the Oswaldo Cruz Foundation in Recife, Brazil (CPqAM/FIOCRUZ).
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