Engineering of vascularized adipose constructs
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Adipose tissue engineering offers a promising alternative to the current surgical techniques for the treatment of soft tissue defects. It is a challenge to find the appropriate scaffold that not only represents a suitable environment for cells but also allows fabrication of customized tissue constructs, particularly in breast surgery. We investigated two different scaffolds for their potential use in adipose tissue regeneration. Sponge-like polyurethane scaffolds were prepared by mold casting with methylal as foaming agent, whereas polycaprolactone scaffolds with highly regular stacked-fiber architecture were fabricated with fused deposition modeling. Both scaffold types were seeded with human adipose tissue-derived precursor cells, cultured and implanted in nude mice using a femoral arteriovenous flow-through vessel loop for angiogenesis. In vitro, cells attached to both scaffolds and differentiated into adipocytes. In vivo, angiogenesis and adipose tissue formation were observed throughout both constructs after 2 and 4 weeks, with angiogenesis being comparable in seeded and unseeded constructs. Fibrous tissue formation and adipogenesis were more pronounced on polyurethane foam scaffolds than on polycaprolactone prototyped scaffolds. In conclusion, both scaffold designs can be effectively used for adipose tissue engineering.
KeywordsAdipose tissue engineering Polycaprolactone Polyurethane Vessel loop Angiogenesis
This work is part of the MD thesis of Paul Wiggenhauser. We thank Professor Dietmar W. Hutmacher for critically reviewing the manuscript and Dr. Hinrich Wiese and Polymaterials AG for generously providing PU foam scaffolds.
- Campa C, Costagliola C, Incorvaia C, Sheridan C, Semeraro F, De Nadai K, Sebastiani A, Parmeggiani F (2010) Inflammatory mediators and angiogenic factors in choroidal neovascularization: pathogenetic interactions and therapeutic implications. Mediators Inflamm. doi: 10.1155/2010/546826
- Exner K (2003) Kapitel 6: Gewebeexpansion. In: Berger A, Hierner R (eds) Plastische Chirurgie - Grundlagen, Prinzipien, Techniken. Springer, BerlinGoogle Scholar
- Eyrich D, Wiese H, Mailer G, Skodacek D, Appel B, Sarhan H, Tessmar J, Staudenmaier R, Wenzel MM, Goepferich A, Blunk T (2007) In vitro and in vivo cartilage engineering using a combination of chondrocyte-seeded long-term stable fibrin gels and polycaprolactone-based polyurethane scaffolds. Tissue Eng 13:2207–2218PubMedCrossRefGoogle Scholar
- Hofer SO, Knight KM, Cooper-White JJ, O'Connor AJ, Perera JM, Romeo-Meeuw R, Penington AJ, Knight KR, Morrison WA, Messina A (2003) Increasing the volume of vascularized tissue formation in engineered constructs: an experimental study in rats. Plast Reconstr Surg 111:1186–1192, discussion 1193–1184PubMedCrossRefGoogle Scholar
- Schantz J-T, Ng KW (2004) A manual for primary human cell culture. World Scientific, SingaporeGoogle Scholar
- Spanholtz TA, Theodorou P, Holzbach T, Wutzler S, Giunta RE, Machens HG (2010) Vascular Endothelial Growth Factor (VEGF(165)) Plus Basic Fibroblast Growth Factor (bFGF) Producing Cells induce a Mature and Stable Vascular Network-a Future Therapy for Ischemically Challenged Tissue. J Surg Res (in press)Google Scholar
- Wiese DH, Maier G, inventors; polyMaterials AG, assignee (2005) Open-Pored Polyurethane Foam without Skin formation, Formulation for the Production thereof and Use Thereof as a Carrier Material for Cell and Tissue Cultures or Medicaments. GermanyGoogle Scholar
- Wiggenhauser PS, Melchels FPW, Hutmacher DW, Machens HG, Ong FR, Schantz JT (2011) Fabrication of a customized tissue engineering scaffold for breast reconstruction. Histol Histopathol 26(supp 1):23Google Scholar