Cell and Tissue Research

, Volume 347, Issue 3, pp 701–711

Scaffold-free culture of mesenchymal stem cell spheroids in suspension preserves multilineage potential

Regular Article


While traditional cell culture methods have relied on growing cells as monolayers, three-dimensional (3D) culture systems can provide a convenient in vitro model for the study of complex cell–cell and cell–matrix interactions in the absence of exogenous substrates and may benefit the development of regenerative medicine strategies. In this study, mesenchymal stem cell (MSC) spheroids, or “mesenspheres”, of different sizes, were formed using a forced aggregation technique and maintained in suspension culture for extended periods of time thereafter. Cell proliferation and differentiation potential within mesenspheres and dissociated cells retrieved from spheroids were compared to conventional adherent monolayer cultures. Mesenspheres maintained in growth medium exhibited no evidence of cell necrosis or differentiation, while mesenspheres in differentiation media exhibited differentiation similar to conventional 2D culture methods based on histological markers of osteogenic and adipogenic commitment. Furthermore, when plated onto tissue culture plates, cells that had been cultured within mesenspheres in growth medium recovered morphology typical of cells cultured continuously in adherent monolayers and retained their capacity for multi-lineage differentiation potential. In fact, more robust matrix mineralization and lipid vacuole content were evident in recovered MSCs when compared to monolayers, suggesting enhanced differentiation by cells cultured as 3D spheroids. Thus, this study demonstrates the development of a 3D culture system for mesenchymal stem cells that may circumvent limitations associated with conventional monolayer cultures and enhance the differentiation potential of multipotent cells.


Mesenchymal stem cell Suspension culture Scaffold-free culture Tissue engineering 

Supplementary material

441_2011_1215_Fig8_ESM.jpg (599 kb)
Supplemental Figure 1

Nile Red staining. Following suspension culture under growth or differentiation conditions, mesenspheres were stained with Nile Red and imaged using a confocal microscope to determine the presence of lipid vacuoles. Optical sections through mesenspheres maintained under growth, adipogenic and osteogenic culture conditions demonstrated the presence of lipid vacuoles throughout adipogenic spheroids following 14 days of differentiation. Some lipid vacuole formation was evident in growth spheroids, while none was evident in osteogenic spheroids. Scale bar 20 μm. (JPEG 598 kb)

441_2011_1215_Fig9_ESM.jpg (641 kb)
Supplemental Figure 2

Maintenance of MSC proliferative capacity following suspension culture. Monolayers and MSCs recovered from mesenspheres were plated onto TCPS and stained with BrdU to confirm the presence of proliferating cells within both populations. BrdU staining indicated a population of actively cycling cells within MSCs recovered from mesenspheres following 7 days of suspension culture (bottom panels). The proportion of BrdU+ cells within recovered cell cultures was comparable to that seen in conventional monolayer cultures (top panels). Scale bar 100 μm. (JPEG 641 kb)

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  1. 1.The Wallace H. Coulter Department of Biomedical EngineeringGeorgia Institute of Technology and Emory UniversityAtlantaUSA
  2. 2.The Parker H. Petit Institute for Bioengineering and BioscienceGeorgia Institute of TechnologyAtlantaUSA
  3. 3.AtlantaUSA

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