Tail regenerative capacity and iNOS immunolocalization in Xenopus laevis tadpoles
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The morphology and the immuno-distribution of the inducible isoform of nitric oxide synthase (iNOS) have been examined in regenerating tails from differently aged Xenopus laevis larvae. By comparing stage-50 and stage-55/56 tadpoles, various morphological aspects and immunoreactivity to anti-iNOS antibody in terms of the number and duration of positive cells have been demonstrated in the regenerating buds. Unlike in stage-50 larvae, the extent of responses to tail amputation in older larvae is more dependent on the individual tadpole and a high percentage (70%-80%) of malformed tails has been seen. The findings indicate that the decline in the efficiency of Xenopus tail regeneration is driven by differences in the inflammatory responses and in the involvement of nitric oxide. This molecule is induced and required for normal tail regeneration, whereas in excess, it is probably associated with progressive loss in the regeneration capability.
KeywordsTadpole Tail regeneration iNOS Immunohistochemistry Xenopus laevis
We thank Prof. Pietro Cardellini (Department of Biology, Padova University, Italy) for supplying sexually mature females and male samples of Xenopus laevis.