Pituitary adenylate cyclase-activating polypeptide type 1 (PAC1) receptor is expressed during embryonic development of the earthworm
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Pituitary adenylate cyclase activating polypeptide (PACAP)-like molecules have been shown to be present in cocoon albumin and in Eisenia fetida embryos at an early developmental stage (E1) by immunocytochemistry and radioimmunoassay. Here, we focus on detecting the stage at which PAC1 receptor (PAC1R)-like immunoreactivity first appears in germinal layers and structures, e.g., various parts of the central nervous system (CNS), in developing earthworm embryos. PAC1R-like immunoreactivity was revealed by Western blot and Far Western blot as early as the E2 developmental stage, occurring in the ectoderm and later in specific neurons of the developing CNS. Labeled CNS neurons were first seen in the supraesophageal ganglion (brain) and subsequently in the subesophageal and ventral nerve cord ganglia. Ultrastructurally, PAC1Rs were located mainly on plasma membranes and intracellular membranes, especially on cisternae of the endoplasmic reticulum. Therefore, PACAP-like compounds probably influence the differentiation of germinal layers (at least the ectoderm) and of some neurons and might act as signaling molecules during earthworm embryonic development.
KeywordsPituitary adenylate cyclase activating polypeptide Western blot Far Western blot Immunocytochemistry Eisenia fetida (Annelida)
- Hernádi L, Pirger Z, Kiss T, Németh J, Márk L, Kiss P, Tamás A, Lubics A, Tóth G, Shioda S, Reglodi D (2008) The presence and distribution of pituitary adenylate cyclase activating polypeptide (PACAP) and its receptor (PAC1-R) in the snail Helix pomatia. Neuroscience 155:387–402CrossRefPubMedGoogle Scholar
- Miyata A, Jiang L, Dahl RD, Kitada C, Kubo K, Fujino M, Minamino M, Arimura A (1990) Isolation of a neuropeptide corresponding to the N-terminal 27 residues of the pituitary adenylate cyclase activating polypeptide with 38 residues (PACAP38). Biochem Biophys Res Commun 170:643–648CrossRefPubMedGoogle Scholar