Cell and Tissue Research

, Volume 338, Issue 2, pp 203–215

Expression of non-neuronal cholinergic system in osteoblast-like cells and its involvement in osteogenesis

  • Maryam En-Nosse
  • Sonja Hartmann
  • Katja Trinkaus
  • Volker Alt
  • Brigitte Stigler
  • Christian Heiss
  • Olaf Kilian
  • Reinhard Schnettler
  • Katrin Susanne Lips
Regular Article

DOI: 10.1007/s00441-009-0871-1

Cite this article as:
En-Nosse, M., Hartmann, S., Trinkaus, K. et al. Cell Tissue Res (2009) 338: 203. doi:10.1007/s00441-009-0871-1

Abstract

Acetylcholine (ACh) is detected in a variety of non-neuronal cells where it acts as a para/autocrine signaling molecule controlling basic cell functions such as proliferation, differentation, and maintenance of cell-cell contacts. ACh-synthesizing enzymes include choline acetyltransferase and carnitine acetyltransferase (CarAT). ACh is released through vesicular exocytosis or directly from the cytoplasm via organic cation transporters (OCT). Extracellular ACh binds to nicotinic (nAChR) and muscarinic receptors (MR). Degradation of ACh is performed by acetylcholinesterase and butyrylcholinesterase (BChE). Here, we have determined whether these molecules are expressed in osteoblast-like cells, by means of reverse transcription polymerase chain reaction and immunohistochemistry, focusing on nAChR subunits α3 and α5. RNA for CarAT, OCT-1, M2R, M5R, nAChR subunits α3, α5, α9, α10, β2, β3, and BChE were detected in human (SAOS-2) and murine (MC3T3-E1) osteoblast-like cells. Other cholinergic components were only expressed species-specifically, e.g., M3R and nAChR subunit α7. Immunhistochemistry localized the nAChR subunits α3 and α5 in osteoblasts in vitro and in vivo where they were up-regulated after application of bone morphogenetic protein-2 (BMP-2) during fracture healing in a rat model. Thus, the cholinergic system of osteoblast-like cells might be regulated by BMP-2 during bone remodeling. Osteoblast-like cells express all necessary enzymes, transporters, and receptors for ACh synthesis and recycling.

Keywords

Non-neuronal cholinergic system Nicotinic receptor Osteoblast Fracture Bone morphogenetic protein-2 Rat (Wistar, adult male) 

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Maryam En-Nosse
    • 1
    • 2
  • Sonja Hartmann
    • 1
  • Katja Trinkaus
    • 1
  • Volker Alt
    • 3
  • Brigitte Stigler
    • 3
  • Christian Heiss
    • 3
  • Olaf Kilian
    • 1
  • Reinhard Schnettler
    • 1
    • 3
  • Katrin Susanne Lips
    • 1
  1. 1.Laboratory for Experimental Trauma SurgeryJustus Liebig UniversityGiessenGermany
  2. 2.Institute for Anatomy and Cell BiologyJustus Liebig UniversityGiessenGermany
  3. 3.Department of Trauma SurgeryJustus Liebig UniversityGiessenGermany

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