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Cell and Tissue Research

, Volume 337, Issue 1, pp 37–43 | Cite as

Characterisation of calcitonin gene-related peptide-immunoreactive neurons in the myenteric plexus of rat colon

  • Retsu MitsuiEmail author
Regular Article

Abstract

A mechanical or chemical stimulus applied to the intestinal mucosa induces motility reflexes in the rat colon. Enteric neurons containing calcitonin gene-related peptide (CGRP) have been suggested as intrinsic primary afferent neurons responsible for mediating such reflexes. In the present study, immunohistochemistry was performed on whole-mount stretch preparations to investigate chemical profiles, morphological characteristics and projections of CGRP-containing neurons in the myenteric plexus of the rat colon. CGRP-positive neuronal cell bodies were detected in preparations incubated with colchicine-containing medium, whereas CGRP-positive nerve fibres were found in colchicine-untreated preparations. These neurons had large oval or round cell bodies that were also immunoreactive for the calcium-binding protein calretinin and neurofilament 200. Myenteric neurons positive for both calretinin and neurofilament 200 had several long processes that emerged from the cell body, consistent with Dogiel type II morphology. Application of the neural tracer DiI to the intestinal mucosa revealed that DiI-labelled myenteric neurons each had an oval or round cell body immunoreactive for calretinin. Thus, CGRP-containing myenteric neurons are Dogiel type II neurons and are immunoreactive for calretinin and neurofilament 200 in the rat colon. These neurons probably project to the intestinal mucosa.

Keywords

Enteric nervous system Intestine Neuropeptide Reflex Immunohistochemistry Rat (male, Wistar) 

Notes

Acknowledgements

The author thanks Prof. Terumasa Komuro (Waseda University) for useful discussions and helpful suggestions.

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  1. 1.Department of Health Science and Social Welfare, Faculty of Human SciencesWaseda UniversityTokorozawaJapan

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