Cell and Tissue Research

, 336:119

Constancy and variability of glomerular organization in the antennal lobe of the silkmoth

  • Tomoki Kazawa
  • Shigehiro Namiki
  • Ryota Fukushima
  • Mitsuhiro Terada
  • Kajin Soo
  • Ryohei Kanzaki
Regular Article

Abstract

We investigated the anatomical organization of glomeruli in the antennal lobes (ALs) of male silkmoths. We reconstructed 10 different ALs and established an identification procedure for individual glomeruli by using size, shape, and position relative to anatomical landmarks. Quantitative analysis of these morphological characteristics supported the validity of our identification strategy. The glomerular organization of the ALs was roughly conserved between different ALs. However, we found individual variations that were reproducibly observed. The combination of a digital atlas with other experimental techniques, such as electrophysiology, optical imaging, and genetics, should facilitate a more in-depth analysis of sensory information processing in silkmoth ALs.

Keywords

Antennal lobe Glomeruli Olfaction Confocal microscopy Digital atlas Bombyx mori (Insecta

Abbreviations

A

anterior

AAT I

anterior antennal lobe tract I

AAT II

anterior antennal lobe tract II

AD

anterior-dorsal

AL

antennal lobe

AV

anterior-ventral

β

β-lobe of the mushroom body

D

dorsal

L

lateral

LAL

lateral accessory lobe

LC

lateral cell cluster

LNs

local interneurons

LT

lateral cell cluster tract

LY

Lucifer yellow

M

medial

MB

mushroom body

MC

medial cell cluster

MGC

macroglomerular complex

MGT

macroglomerular tract

MT

medial cell cluster tract

OACT

outer antenno-cerebral tract

OB

olfactory bulb

OG

ordinary glomeruli

ORN

olfactory receptor neuron

P

posterior

PAT I

posterior antennal lobe tract I

PD

posterior-dorsal

PN

projection neuron

V

ventral

Supplementary material

441_2009_756_Fig1_ESM.gif (895 kb)
Supplementary Fig. 1

Variation of glomeruli observed by anti-synaptotagmin antibody staining. Confocal images of ALs stained by synaptotagmin antibody confirmed the variations of glomeruli. a, b Confocal images around VLG, VCG1, and VCG2 in the AV region. A small glomerulus (dotted line in b), emerged shown in b but no in a. The depth of the optical slices from the top of AL is shown upper right. c, d Confocal images of DLLeaf and adjacent glomeruli in the ALs in which DLLeaf was not divided (c) and was divided into two glomeruli (d). DLLeaf in c and the two separated glomeruli within DLLeaf in d are marked by dotted lines (D dorsal, L lateral). Bar 50 µm (GIF 984 KB)

441_2009_756_Fig1_ESM.eps (9.9 mb)
High resolution image file (EPS 9.86 MB).
441_2009_756_MOESM3_ESM.pdf (5 kb)
ESM 1(PDF 5.16KB)

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Tomoki Kazawa
    • 1
  • Shigehiro Namiki
    • 1
    • 2
  • Ryota Fukushima
    • 1
    • 2
  • Mitsuhiro Terada
    • 2
  • Kajin Soo
    • 2
  • Ryohei Kanzaki
    • 1
  1. 1.Research Center for Advanced Science and TechnologyUniversity of TokyoTokyoJapan
  2. 2.Graduate School of Life and Environmental SciencesUniversity of TsukubaTsukubaJapan

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