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Cell and Tissue Research

, 335:565 | Cite as

Gene and microRNA expression signatures of human mesenchymal stromal cells in comparison to fibroblasts

  • Sohyun Bae
  • Jung Hoon Ahn
  • Chae Woon Park
  • Hye Kyung Son
  • Keun-Soo Kim
  • Nam-Kyu Lim
  • Choon-Ju Jeon
  • Hoeon KimEmail author
Regular Article

Abstract

Human mesenchymal stromal cells (MSCs) offer great hope for the treatment of tissue degenerative and immune diseases, but their phenotypic similarity to dermal fibroblasts may hinder robust cell identification and isolation from diverse tissue harvests. To identify genetic elements that can reliably discriminate MSCs from fibroblasts, we performed comparative gene and microRNA expression profiling analyses with genome-wide oligonucleotide microarrays. When taken globally, both gene and microRNA expression profiles of MSCs were highly similar to those of fibroblasts, accounting well for their extensive phenotypic and functional overlaps. Scattered expression differences were pooled to yield an MSC-specific molecular signature, consisting of 64 genes and 21 microRNAs whose expressions were at least 10-fold and two-fold higher, respectively, in MSCs compared with fibroblasts. Genes either encoding transmembrane proteins or associated with tumors were relatively abundant in this signature. These data should provide the molecular basis not only for the discovery of novel diagnostic markers discriminating MSCs from fibroblasts, but also for further studies on MSC-specific signaling mechanisms.

Keywords

Gene expression profile MicroRNA profile Mesenchymal stromal cells Fibroblasts 

Supplementary material

441_2008_729_MOESM1_ESM.doc (112 kb)
Supplementary Table 1 Gene expression signature of MSCs in comparison with fibroblasts (DOC 111 KB)
441_2008_729_MOESM2_ESM.xls (64 kb)
Supplementary Table 2 Hybridization intensity ratio of microRNAs (XLS 64 KB)

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Sohyun Bae
    • 1
  • Jung Hoon Ahn
    • 2
  • Chae Woon Park
    • 1
  • Hye Kyung Son
    • 1
  • Keun-Soo Kim
    • 3
  • Nam-Kyu Lim
    • 1
  • Choon-Ju Jeon
    • 1
  • Hoeon Kim
    • 1
    Email author
  1. 1.Biotherapeutic DivisionGenexel-Sein Inc.DaejonKorea
  2. 2.Korea Science AcademyBusanKorea
  3. 3.Antibody Engineering Research Unit, KRIBBDaejonKorea

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