Cell and Tissue Research

, Volume 332, Issue 3, pp 469–478 | Cite as

Synovial mesenchymal stem cells accelerate early remodeling of tendon-bone healing

  • Young-Jin Ju
  • Takeshi Muneta
  • Hideya Yoshimura
  • Hideyuki Koga
  • Ichiro SekiyaEmail author
Regular Article


Tendon-bone healing is important for the successful reconstruction of the anterior cruciate ligament by using the hamstring tendon. Mesenchymal stem cells (MSCs) have attracted much interest because of their self-renewing potential and multipotentiality for possible clinical use. We previously reported that MSCs derived from synovium had a higher proliferation and differentiation potential than the other MSCs that we examined. The purpose of this study was to investigate the effect and mechanism of the implantation of the synovial MSCs on tendon-bone healing in rats. Half of the Achilles’ tendon grafts of rats were inserted into a bone tunnel from the tibial plateau to the tibial tuberosity with a suture-post fixation. The bone tunnel was filled with MSCs labeled with fluorescent marker DiI or without MSCs as the control. The tendon-bone interface was analyzed histologically, and collagen fibers were quantified. At 1 week, the tendon-bone interface was filled with abundant DiI-positive cells, and the proportion of collagen fiber area was significantly higher in the MSC group than in the control group. By 2 weeks, the proportion of oblique collagen fibers, which appeared to be Sharpey’s fibers, was significantly higher in the MSC group than in the control group. At 4 weeks, the interface tissue disappeared, and the implanted tendon appeared to attach to the bone directly in both groups. DiI-labeled cells could no longer be observed. Implantation of synovial MSCs into bone tunnel thus accelerated early remodeling of tendon-bone healing, as shown histologically.


Mesenchymal stem cells Tendon-bone healing Anterior cruciate ligament reconstruction Cell therapy Synovium Rat (Sprague Dawley) 



We thank Kenichi Shinomiya, MD, PhD, for continuous support and Miyoko Ojima for expert help with histology. Recombinant human BMP2 was kindly provided by Astellas Pharma.


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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Young-Jin Ju
    • 1
  • Takeshi Muneta
    • 1
    • 2
  • Hideya Yoshimura
    • 1
  • Hideyuki Koga
    • 1
  • Ichiro Sekiya
    • 3
    Email author
  1. 1.Section of Orthopedic Surgery, Graduate SchoolTokyo Medical and Dental UniversityTokyoJapan
  2. 2.Center of Excellence Program for Frontier Research on Molecular Destruction and Reconstruction of Tooth and BoneTokyo Medical and Dental UniversityTokyoJapan
  3. 3.Section of Cartilage Regeneration, Graduate SchoolTokyo Medical and Dental UniversityTokyoJapan

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