Formation of giant spicules in the deep-sea hexactinellid Monorhaphis chuni (Schulze 1904): electron-microscopic and biochemical studies
The siliceous sponge Monorhaphis chuni (Hexactinellida) synthesizes the largest biosilica structures on earth (3 m). Scanning electron microscopy has shown that these spicules are regularly composed of concentrically arranged lamellae (width: 3–10 μm). Between 400 and 600 lamellae have been counted in one giant basal spicule. An axial canal (diameter: ~2 μm) is located in the center of the spicules; it harbors the axial filament and is surrounded by an axial cylinder (100–150 μm) of electron-dense homogeneous silica. During dissolution of the spicules with hydrofluoric acid, the axial filament is first released followed by the release of a proteinaceous tubule. Two major proteins (150 kDa and 35 kDa) have been visualized, together with a 24-kDa protein that cross-reacts with antibodies against silicatein. The spicules are surrounded by a collagen net, and the existence of a hexactinellidan collagen gene has been demonstrated by cloning it from Aphrocallistes vastus. During the axial growth of the spicules, silicatein or the silicatein-related protein is proposed to become associated with the surface of the spicules and to be finally internalized through the apical opening to associate with the axial filament. Based on the data gathered here, we suggest that, in the Hexactinellida, the growth of the spicules is mediated by silicatein or by a silicatein-related protein, with the orientation of biosilica deposition being controlled by lectin and collagen.
KeywordsSpicules Silica formation Silicatein-related protein Hexactinellida Monorhaphis chuni (Porifera)
The phylum Porifera (sponges) has been subdivided into three classes, viz., the Hexactinellida, Demospongiae, and Calcarea, whereby the first two taxa comprise individuals with a siliceous skeleton composed of spicules, and members of the last-mentioned taxon have calcareous (calcium carbonate) skeletal elements. Based on molecular biological (Kruse et al. 1997) and geological (Reitner and Wörheide 2002) studies, the Hexactinellida taxon has been established as the oldest, having evolved between Neoproterozoic glaciations (720–585 Ma), sometimes referred to as “snowball” Earth (for a review, see Corsetti et al. 2006). During this period, a cycle of silicate weathering and carbonate precipitation, prior to or simultaneously with the glaciations, has been proposed to have resulted in the dissolution of surface rocks composed of insoluble silicates (CaSiO3). Subsequently, soluble calcium carbonate (CaCO3) and soluble silica (SiO2) was formed by reaction with atmospheric CO2 (Walker et al. 1981). In consequence, the level of dissolved silicic acid in seawater increased providing the inorganic starting material for the formation of siliceous spicules. Physical and chemical analyses have revealed that the siliceous skeletons of the two siliceous sponge classes are composed of amorphous silica with a water content of around 10% (Sandford 2003). Analysis of infrared spectra have shown a distinct difference between the two taxa of between 1,080 and 1,100 cm−1 suggesting a different molecular configuration involving the Si-O-Si linkage (Sandford 2003). Other than silicic acid, only small amounts of trace elements (<3%) are found in the spicules.
During the past few years, some aspects of the mechanism of spicule formation in Demospongiae have been clarified. Work mainly with Tethya aurantium (Shimizu et al. 1998; Cha et al. 1999) and Suberites domuncula (Krasko et al. 2000) has established that, in contrast to silica deposition in other organisms (e.g., in diatoms), biosilica formation in sponges is mediated enzymatically via the enzyme silicatein. Silicatein forms the axial filament of the spicules from which the growth of the spicules starts intracellularly (Shimizu et al. 1998; Müller et al. 2003, 2005; Wang and Wang 2006). Although marine sponges contain two isoforms of silicatein at least, freshwater sponges contain up to five silicatein isoforms, indicating the more complex formation and organization of the spicules in these animals (Müller et al. 2006). In S. domuncula, and probably also in other Demospongiae, spicule formation starts intracellularly. After reaching a size of approximately 6–8 μm, the macroscleres (spicules of >10 μm) are extruded from the cells (sclerocytes) and completed extracellularly, where they may reach lengths of over 100 μm (Müller et al. 2005). In the extracellular space, both the final longitudinal size and the final thickness of the spicules are reached by appositional growth (Müller et al. 2006). These processes are mediated by silicatein and controlled by galectin, which acts as an organic matrix for the attachment of silicatein molecules (Schröder et al. 2006). Collagen probably provides the structural groove along which the galectin-silicatein strings are guided, thus allowing the formation of the intricately architectured spicules (Eckert et al. 2006).
The monophyletic group of Hexactinellida is, according to the form and organization of its spicules, divided into two subclasses: the Hexasterophora and Amphidiscophora (for a review, see Reiswig 2006). In the latter subclass, amphidiscs are the (main) microscleres and never fuse. One member of this subclass is the family Monorhaphididae, which includes three described species: Monorhaphis chuni, M. dives, and M. intermedia. Since the discovery of these sponges during the first German deep-sea expedition (RV “Valdivia”) and the first descriptions of these animals by Chun (1900) and Schulze (1904), only little additional information has been added regarding the form and construction of their skeletal systems. Nothing has been published concerning the synthesis of their giant spicules. Worldwide, M. chuni has been documented only at a few sampling sites (Tabachnick 2002), suggesting a distribution in the deep sea in the Indian Ocean and in the West Pacific. One outstanding feature of Monorhaphis is its anchoring spicule, which can reach lengths of up to 3 m with a maximum diameter of 8.5 mm (Schulze 1904). M. chuni thus produces the largest biosilica structure known on Earth. Polished cross sections confirm the existence of up to 500 highly regular concentric rings that are arranged around a 150-μm-thick less-structured core (Schulze 1904). In the middle of this core, a square axial channel with a proteinaceous filament of approximately 2 μm in thickness is visible running through the entire spicule from one end to the other. The concentric rings around this core have nearly constant thickness in all samples, but this thickness varies among sampling locations; for example, from the Somalia basin, silica strata are 8–10 μm in thickness, whereas those from the Madagascar basin are 3–5 μm thick.
Recently, Aizenberg et al. (2005) have published a detailed structural analysis of the spicule formation in the hexactinellid Euplectella. They have demonstrated structural hierarchies of spicule synthesis starting from the nanometer-sized particles of silica to the final mature spicules. The composition of the proteins that are associated with and found within the spicules has not been described. In a recent study, Ehrlich et al. (2005) dissolved the basal spicules of the hexactinellid Hyalonema sieboldi in 2.5 M NaOH at 30°C for 14 days and were able to show that the abundant structural protein associated with the spicules was probably collagen with an Mr of around 130 kDa. However, whether degradation of the collagen fibrils occurred during the dissolution of silica with NaOH remained unclear. In continuation of these studies, we have analyzed the protein composition of the spicule-associated protein(s) in the spicules of M. chuni and M. intermedia; the results are presented here.
We present a microscopic analysis of the spicules from M. chuni with major emphasis on the giant spicules (giant basal spicules or basalia: 1 m) and also the large comitalia (~60 mm). This is followed by an examination of the organic components of these spicules, including the collagen fibrils that surround these spicules. Data obtained from electron microprobe analysis are given that show the regionally different composition of sodium and potassium within the spicules. After dissolution of the spicules, several proteins have been visualized: one cross-reacts with antibodies raised against silicatein, whereas others display proteolytic activity. After having substantiated, by molecular biological techniques, that Hexactinellida possess at least one gene coding for collagen (cDNA from the hexactinellid Aphrocallistes vastus), we provide a schematic outline of spicule growth, both in the longitudinal and the axial direction.
Materials and methods
Enzymatic CarboRelease kit (No. KEDG-01) was obtained from QA-Bio (Novatec, Basel, Switzerland); electrophoresis loading buffer (RothiLoad4) from Roth (Karlsruhe, Germany); Sypro Ruby protein gel stain from BioRad (München, Germany); casein and Coomassie Brilliant Blue from Serva (Heidelberg, Germany); Sirius Red [3BA], tetraethoxysilane (TEOS), and Rhodamine 123 from Sigma-Aldrich (Taufkirchen, Germany); horse blood from Fiebig Nährstofftechnik (Germany); and GelCode blue from Perbio (Bonn, Germany).
For the isolation of the cDNA encoding collagen, a cDNA library of Aphrocallistes vastus (Hexactinellida:Hexasterophora:Hexactinosida:Aphrocallistidae) was used (Gundacker et al. 2001).
Extracts from spicules of M. chuni, S. domuncula, and G. cydonium for the determination of proteolytic activity in the spicules were prepared as described by Schröder et al. (2006) and involved grinding the spicules in TRIS-buffered saline pH 7.5 (TBS) with 1 mM EDTA, 1% Nonidet-P40, supplemented with 4 M urea. After centrifugation, the supernatant was used for zymogram analysis.
Scanning electron microscopy (SEM) analysis of spicules was performed with a Zeiss DSM 962 Digital Scanning Microscope (Zeiss, Aalen, Germany). The samples were mounted onto aluminum stubs (SEM-Stubs G031Z; Plano, Wetzlar, Germany) that had been covered with adhesive carbon (carbon adhesive Leit-Tabs G3347). These preparations were then sputtered with a 20-nm layer of gold in an Argon plasma (Bal Tec Med 020 coating system; Bal Tec, Balzers, Liechtenstein). The surfaces of spicule cross sections had been polished with emery paper (silicon carbide; Matador, Hoppenstedt, Darmstadt, Germany), the quality of the surface having been inspected under a stereomicroscope (enlargement: about 30×). Backscattered analysis was performed with this microscope and by using 15-KeV beam voltage and 50-μA emission current at a working distance of 6 mm (Holmes et al. 1987).
Electron microprobe procedure
Thin sections (150 μm) of giant basal spicules were prepared and subjected to electron microprobe analyses as described by Sommer et al. (2003). Mineral analyses were performed by means of a JOEL 8900RL Superprobe device attached to a MICROSPEC wavelength dispersive system. Matrix correlations for silicates were achieved by using the ZAP procedure and natural mineral standards.
The sequential release of the organic material from the giant basal spicules collected in 1898 (Schulze 1904) was analyzed as described previously (Müller et al. 2006). The spicules still contained residual layers of surface collagen-like sheaths. Spicules were placed onto glass slides and etched by being covered with 4 M hydrofluoric acid (HF). To visualize the released proteins, Coomassie solution [0.1% (w/v) Coomassie Brilliant Blue 250, 40% methanol, 10% acetic acid] was added. Photographs were taken at various times of incubation (up to 40 min) via an Olympus AHBT3 microscope.
Tissue from M. chuni was dissolved in sulfuric/nitric acid (3:1), and the spicules were washed with distilled water. For the complete dissolution of silica from the cleaned spicules, they were treated with 2 M HF, 8 M NH4F, pH 5 (Shimizu et al. 1998) at room temperature overnight. The axial filaments and the associated proteins were collected by centrifugation (20,000g, 10 min, 4°C). The sediment was dialyzed against distilled water as described by Shimizu et al. (1998).
Where indicated the spicules were stained with Sirius Red for collagen as described by Junqueira et al. (1979).
SDS-polyacrylamide gel electrophoresis and Western blot analysis
Protein samples (25 μg) were taken from the axial filament after complete dissolution of the spicules with HF, dissolved in loading buffer (Roti-Load; Roth, Karlsruhe, Germany), boiled for 5 min, and then subjected to 10% polyacrylamide gel electrophoresis (PAGE) with 0.1% sodium dodecyl sulfate (SDS-PAGE). After separation, the gels were washed in 10% methanol (supplemented with 7% acetic acid) for 30 min, stained in Sypro Ruby protein gel stain overnight, and finally inspected under ultraviolet light (excitation wavelength: 302 nm).
For Western blot analysis, the polypeptides were transferred to polyvinylidene fluoride (PVDF) membranes (Millipore-Roth, Karlsruhe; Germany) and reacted with polyclonal antibodies (PoAb) raised against silicatein from S. domuncula as described earlier (termed PoAb-aSILIC; Müller et al. 2005). The PVDF membranes were then rinsed in TBS-T (20 mM TRIS-HCl pH 7.6, 137 mM NaCl, 0.1% Tween-20) and incubated for 1 h with PoAb-aSilic (1:1,000 dilution) diluted in TBS-T supplemented with 5% non-fat dry milk and 1.5% bovine serum albumin. Membranes were washed three times in TBS-T and then incubated for 1 h with anti-rabbit IgG (alkaline phosphatase conjugate; 1:2,000 dilution; Sigma). Immunocomplexes were visualized with the NBT/BCIP color development system (Roth; nitroblue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate). For controls, we used pre-adsorbed PoAb-aSILIC (100 μl antibodies were incubated with 20 μg recombinant silicatein rSILIC_ SUBDO). This antibody preparation gave no signals in Western blots (Schröder et al. 2006). A protein size-standard (Dual Color; Roth) was used to estimate the size of the proteins.
Deglycosylation of proteins of giant basal spicules
Total extracts from spicules after dissolution of silica by HF were subjected to enzymatic deglycosylation by applying the CarboRelease reaction kit. The proteins of the spicules dissolved in 50 mM Na-phosphate (pH 7.0) were denatured by incubating the samples at 95°C for 5 min. Subsequently, the samples were incubated at 37°C for 3 h with the enzyme cocktail of the CarboRelease reaction kit (PNGase), which consisted of N-glycosidase F (Chryseobacterium meningospeticum; specific activity: 5 mU), O-glycosidase (Streptococcus pneumoniae; 1.25 mU), sialidase (Arthrobacter ureafaciens; 5 mU), β-galactosidase (S. pneumoniae; 50 mU), and glucosaminidase (S. pneumoniae; 1 mU). The reaction products were then analyzed by electrophoresis by SDS-PAGE as described above.
Analysis of proteolytic activity in spicular extracts
Proteins were released from giant basal spicule after complete HF dissolution and examined for protease activity by zymogram analysis. Prior to this procedure, the extract was dialyzed against phosphate-buffered saline and subsequently loaded onto the zymogram gel, which contained 0.1% casein, essentially as described by Jaffe and Dwyer (2003). The samples were separated by SDS-PAGE (12% gels). Samples of 15 μl were loaded onto each gel, corresponding approximately to a protein extract from 400 μg spicules. After separation, the gels were incubated in renaturation buffer (50 mM MOPS pH 6.8, 5 mM CaCl2, 0,1 mM ZnCl2, 100 mM NaCl, 0,5 mM dithiothreitole) for 1 h at room temperature. After this buffer had been refreshed, protease activity was allowed to develop overnight at 37°C. The gels were then stained with Coomassie Brilliant Blue in order to visualize protease activity as clear bands on a blue background.
The following samples were tested: extracts from spicules of M. chuni and from the Demospongiae G. cydonium and S. domuncula. In one series of experiments, the M. chuni extract was heated at 95°C for 5 min prior to application onto gels.
Cloning of cDNA for collagen from A. vastus
The complete collagen [COL_APHRO], APHVACOL, was isolated from a cDNA library of A. vastus, which was prepared in lamda ZAP Express and packaged in vitro with the MaxPlaxTM Packaging Extract as described by Gundacker et al. (2001). A degenerate forward primer was designed against the collagen-specific amino acid (aa) triplets. The polymerase chain reaction (PCR) was carried out with this primer and a vector primer at an initial denaturation at 95°C for 5 min, followed by 35 amplification cycles at 95°C for 30 s, 52°C for 45 s, and 71°C for 1.5 min, with a final extension step at 74°C for 10 min. Fragments were obtained and cloned into the TOPO TAII vector in Escherichia coli TOP10 cells. Sequencing was performed with primers directed to the SP6 promoter (5′-ATTTAGGTGACACTATAG-3′) and the T7 promoter (5′-TAATACGACTCACTATAGGG-3′). The sequence was completed with insert-specific primers in combination with 5′-RACE (rapid amplification of cDNA ends) primer (5′-GTCTACCAGGCATTCGCTTCAT-3′) or with 3′-RACE primer (5′-CTGTGAATGCTGGACTACGAT-3′) by using the CapFishing Full-length cDBA Premix Kit. The final sequence was confirmed by an additional PCR with primers directed against the non-translated region of the cDNA, followed by sequencing. The clone encoding A. vastus collagen was 2,610 nucleotides (nts) long, excluding the poly(A) tail.
The sequences were analyzed with computer programs BLAST (2005; http://www.ncbi.nlm.nih.gov/blast/blast.cgi) and FASTA (2005; http://www.ebi.ac.uk/fasta33/). Multiple alignments were performed with CLUSTAL W Ver. 1.6 (Thompson et al. 1994). Phylogenetic trees were constructed on the base of aa-sequence alignments by neighbor-joining, as implemented in the Neighbor program from the PHYLIP package (Felsenstein 1993). The distance matrices were calculated by using the Dayhoff PAM matrix model as described (Dayhoff et al. 1978). The degree of support for internal branches was further assessed by bootstrapping (Felsenstein 1993). The graphic presentations were prepared with GeneDoc (Nicholas and Nicholas 1997).
The Bradford method (Compton and Jones 1985; Roti-Quant solution, Roth) was used for the quantification of protein.
Spicule organization of M. chuni
The largest spicules in the body of M. chuni are the comitalia, the parenchymal diactin spicules. The comitalia are located in the choanosome and reach lengths up to 60 mm and diameters of 100 μm (Fig. 2d). The surface of the comitalia is covered by a net of collagen, which is stained red with the Sirius Red stain. Tauactin spicules are arranged around the comitalia (Fig. 2d). At distances of approximately 200 μm, the comitalia show central swellings within the lamellar arrangement of the silica layers. In these swellings, cryptic silica structures can be visualized that comprise hexactin structures (Fig. 2e). Like the other spicules, the comitalia are also composed of lamellar layers of silica arranged around the 2– to 4-μm-thick axial canal (Fig. 2f).
Lamellar layer organization of comitalia and basalia
The organization and arrangement of the silica layers of the comitalia are similar to the concentric layering of the giant basal spicules (basalia). Whereas the basalia reach sizes of up to 3 m, the comitalia are relatively short at 60 mm (see also above).
The inter-lamellar zone of the spicules is surprisingly not formed by a continuous open slit (Fig. 3g). The inter-lamellar zone (width: 0.1–0.2 μm) is composed of open holes and fusion zones that allow a continuum between two silica lamellae (Fig. 3h,i). This structural organization is seen more frequently between the lamellae of the spicules.
Arrangement of collagen layers around spicules
Electron microprobe analysis
Wavelength-dispersion mapping with an electron microprobe also indicated the high concentration of Si throughout the spicule (Fig. 6). For K, the level was higher at the central part and dropped drastically at the rim of the spicule (Fig. 6). An inverted distribution was measured for Na: low at the center and high at the surface of the spicules (Fig. 6).
Analysis of proteins contained in spicules
Deglycosylation of spicular proteins and Western blot analysis
The proteins released from the giant basal spicules were deglycosylated enzymatically and likewise separated by SDS-PAGE. The most obvious shift in the size of the protein band was seen for the 35-kDa polypeptide band (prior to deglycosylation), which showed an apparent size of 30 kDa after deglycosylation (Fig. 8a; lane b). After this treatment, the 24-kDa polypeptide remained in the preparation (Fig. 8a; lane b).
In order to obtain a first indication of whether silicatein was present in the native preparation from spicules of M. chuni, the proteins were subjected, after SDS-PAGE, to Western blot analysis. After blot transfer, the proteins were reacted with PoAb-aSilic and subsequently with an alkaline-phosphatase-labeled secondary antibody. After this procedure, a clear band corresponding to 24 kDa became visible (Fig. 8b; lane a). This polypeptide did not react with PoAb-aSilic that had been pre-treated with recombinant silicatein (control; not shown).
Proteolytic activity in spicular extracts
Cloning of cDNA for collagen from an hexactinellid
The highest similarity score of the A. vastus collagen was determined with the type IV collagen alpha 1 chain precursor from Hydra vulgaris, showing a “expect value” (Coligan et al. 1998) of 4×e−42. Similar high scores existed for other metazoan collagen sequences. For the phylogenetic analysis, the sequences with the highest similarities to the A. vastus collagen were selected not only from Hydra, but also from human (alpha 1 chain-like collagen precursor), Drosophila melanogaster (collagen type IV), Caenorhabditis elegans (collagen alpha-2 chain precursor), and the two S. domuncula sequences (collagen1 and collagen2; Fig. 10b). No collagen sequences were identified in non-metazoan organisms. After alignment of these sequences, the slanted tree was constructed and rooted with the A. vastus sequence as the outgroup. The Hydra sequence fell into one branch, together with the collagens from the protostomians D. melanogaster and C. elegans, whereas the two collagens from S. domuncula clustered together with the human sequence (Fig. 10b).
At present, the marine environment is relatively poor in silicon concentration, ranging from 3 μM at the surface to 180 μM in deep water (Maldonado et al. 1999). However, sponges are provided, as has been shown in the siliceous demosponge S. domuncula, with a transporter system for silica uptake; this probably involves a Na+/HCO3 −[Si(OH)4] (NBCSA) cotransporter (Schröder et al. 2004a). The higher concentration of silicon/silica in the deep sea can be postulated as facilitating and allowing the formation of giant siliceous sponges, with the 3-m-high M. chuni being a good example. The major skeletal elements of these sponges are the giant basal spicules that reach a size of 3 m and a diameter of up to 7 mm (see above). These large spicules might allow the animals to maintain a siutable distance from the muddy ground on which they live (Schulze 1904). Stony corals are sometimes found on these spicules (Schulze 1904) suggesting a mutualistic (at least) symbiotic relationship. Recent molecular biological studies have revealed that the siliceous demosponges are provided with the genetic “toolkit” to construct these skeletal elements, the spicules, functional animals being composed of differentiated cells that interact in a tissue-like manner and that allow the formation of a body plan (Müller et al. 2004; Müller 2005; Pilcher 2005). M. chuni is a siliceous sponge, belonging to the phylogenetically oldest Poriferan class, the Hexactinellida (Kruse et al. 1997).
The structure of hexactinellidan spicules has previously been studied to some considerable detail, most notably by Schulze (1904) and Schmidt (1926) who, in the early literature, reported the lamellar organization of the silica layers of the spicules; these layers have been assumed to be separated from each other by organic components (Schmidt 1928). Levi et al. (1989) have demonstrated that this lamellar organization contributes significantly to the stabilization of the giant basal spicules of Monorhaphis. Only recently successful attempts have been performed to clarify, from a structural biological point of view, the formation of spicules in the hexactinellid Euplectella (Aizenberg et al. 2005). Ehrlich et al. (2005) obtained the first indications that collagen contributes to the formation of hexactinellidan spicules. In the present study, we have investigated the morphology and formation of the spicules from M. chuni, mainly focusing on the large comitalia and the giant basal spicules.
The lamellar organization, both of the smaller (choanosomal) spicules and the larger comitalia and giant basal spicules, have been studied by using 100-year-old samples and recent spicules. In these two types of spicules, the size of the lamellae is approximately 5 μm in width. The lamellae are separated from each other by a gap that shows, in the more central regions, no continuum but concentrically arranged holes separated by silica bridges, allowing the lamellae to fuse. These silica bridges have been seen in all spicules analyzed, strongly suggesting that they are not formed secondarily. In the central core of the spicule, the lamellae are fused together appearing as a solid silica cylinder, a pattern that is especially pronounced in the giant basal spicules. This cylinder/canal harbors the axial canal with its axial filament. As shown in this study, the immature growing spicules exhibit a rectangular axial canal, suggesting a similar shape for the axial filaments. This form of the canal suggests that the filamentous proteins in M. chuni are arranged in a tetragonal pattern. A hexagonal organization of the silicatein in the axial filaments has been demonstrated in the demosponge G. cydonium (Croce et al. 2004). However, detailed studies of hexactinellids are lacking. We have adopted the view of Schulze (1904), namely that the central cylinder, which reaches a width of 100–150 μm is also initially synthesized in a lamellar manner and not as a solid stalk, taking into account that the enzymatic synthesis of the lamellae in Demospongiae never exceeds a width of 20 μm. The axial filament in the spicules can, with time and growth of the spicules, become thinner, as has long been known (Schmidt 1926). The number of lamellae in the giant basal spicules of M. chuni can attain 400–600. The thickness of the lamellae within the spicules in M. chuni varies between 3 and 10 μm. The axial canals in growing spicules from M. chuni are larger (3 to 5 μm) than those found in completed spicules, as previously mentioned by Schulze (1904). In the spicules, the canal can even disappear and become filled with silica (Schulze 1904). The major reason for studying the structure of the spicules here has been to understand the growth pattern of the spicules in an axial and radial direction. All (or almost all) spicules of M. chuni are open at their tips (Schulze 1904; Uriz 2006; Uriz et al. 2003); their axial canals occasionally expose the axial filaments. This finding has contributed to the understanding of the axial growth of the spicules (see below). The data available indicate that all spicules in Hexactinellida possess an axial filament (Uriz 2006).
The initial chemical composition of hexactinellidan spicules was provided by Schulze (1904), who found that, other than Si (96%), only Na and K contribute to the inorganic material in measurable amounts. This composition was later confirmed by Levi et al. (1989) and Sandford (2003). In the analytical data, presented here, Si has been shown to be uniformly distributed throughout the silica shell of the spicules, whereas Na and K are not. Higher levels of K have been measured (around 1 wt%) in the central part of the spicules, whereas the amount drops considerably (≈0.4 wt%) at the surface. The inverse is true of the distribution of Na; here, the level is almost negligible at the center (≈0.03 wt%) but increases toward the surface to ≈0.4 wt%. One explanation might be that the much higher concentration of Na than K in seawater (Kennish 1994) causes an exchange to occur with respect to the initially synthesized K+-silicate to Na+-silicate, after exposure of the siliceous spicules to the surrounding milieu. However, another explanation might also be possible: Iler (1979) and Perry (2003) have demonstrated that, in the presence of smaller cations (e.g., Na+), a faster condensation rate of silica occurs, in comparison with the rate measured with larger cations (e.g., K+). This view is supported by studies with diatoms in which Si transport into cells has been proposed to be driven by Na+-gradients generated and maintained across the membrane by the activity of a Na+/K+-ATPase (Sullivan 1986). In sponges, with S. domuncula as a model, data have been presented that are compatible with the view that silica uptake is mediated by an NBCSA cotransporter. If this assumption reflects the physiological situation, then Na+-silicate condensation products would first be formed at the sites of synthesis of the spicules during the appositional layering of silica lamella that occurs on the outer surface of the spicules and would be converted to K+-silicate containing oligo- and polymers. This argument is sustained by results of a previous study: (1) in other preparations from M. chuni (Levi et al. 1989), an overall high concentration of sodium exists in these spicules (approximately 50% of the concentration determined for potassium), and (2) an apparent uniform distribution of these two ions occurs throughout the spicules (Levi et al. 1989). The zonation of lamellae with different mechanical properties has been proposed by Schulze (1904) and Schmidt (1926) and, more recently, by Levi et al. (1989). However, no data concerning the water content or the hardness of the various layers have existed until now. The differential chemical composition within the spicules, as described here, can be considered as a first step toward an understanding of their mechanical properties. Additionally, we have performed spectroscopical analysis to identify water in the amorphous silica (not shown here) and have confirmed the previous data of Levi et al. (1989), indicating that M. chuni spicules contain between 5% and 10% water in combination with silicate.
In all Demospongiae, the formation of the spicules starts from the axial canal, followed by an appositional layering of the silica lamellae (Schröder et al. 2006). Two methods have been employed to localize the protein(s) within the spicules of M. chuni; first, by dissolution with HF and simultaneous staining with Coomassie Brilliant Blue, and second, by biochemical techniques. Until now, (almost) no biochemical data has been available with regard to the spicules of hexactinellidan sponges. Here, we show, by a stepwise dissolution of the siliceous lamella from the spicules, that the axial filament is released. The dissolution of the silica shell around the axial filament does not occur uniformly, but rather in a zigzag manner apparently along the slits between two lamellae. We have also demonstrated that, after exposure of the axial filament and following completion of the dissolution of the inorganic material, a proteinaceous tube remains that has the diameter of the outer surface of the spicules. Since no evidence from electron-microscopic images has been obtained concerning the existence of a continuous protein layer within the inner layers of the spicules, we assume that this tube represents the matrix of the silicification process of the outer lamellae. In previous studies, proteinaceous filaments have been assumed to traverse the lamellae (see Schmidt 1926). However, all our electron-microscopic studies and the light-microscopic images favor the view that, other than the inner axial filament, the organic material of the spicules is concentrated in the proteinaceous tube surrounding the external surface of the spicules. Hence, no structural bundles are expected to be located within these silica lamellae.
After complete dissolution of the silica shell of the giant basal spicule by HF, the residual protein “shell” was analyzed by SDS-PAGE. The results were informative in several aspects. First, two dominant protein molecules could be identified, having a size of 150 kDa and 35 kDa. In previous studies with Demospongiae, no high-molecular-weight protein could be obtained after dissolution with HF (Schröder et al. 2004b); high-molecular-weight polypeptides of 250 kDa could only be identified after a less harsh release of the proteins from the spicules, viz., by mechanical grinding and urea extraction (Schröder et al. 2006). No data on the function of this large protein (250 kDa) exist. The 35-kDa protein species identified in M. chuni is likewise present in S. domuncula; in the latter demosponge, this protein is a galectin. Recent studies with M. chuni extracts have revealed the presence of a lectin (data not shown here). This 35-kDa protein has been identified as a glycoprotein that, after deglycosylation, migrates as a molecules of 30 kDa. This is the first published data regarding the participation of glycoproteins in the construction/synthesis of siliceous spicules. As a result of this finding, parallel experiments have been performed with S. domuncula spicules and have likewise revealed the existence of glycoproteins (to be published). Finally, one protein species with a molecular weight of 24 kDa should be mentioned because, on the basis of its size, it might be silicatein (Schröder et al. 2004b, 2006). Application of antibodies raised against S. domuncula silicatein has shown cross-reactivity to the 24-kDa M. chuni protein. This finding could be taken as the first evidence for the existence of silicatein (or of a silicatein-related protein) in Hexactinellida.
In a recent study, a model for the appositional growth of the spicules from Demospongiae has been proposed that suggests the involvement of silicatein and additional proteins, e.g., galectin, which might mediate silica deposition in a centrifugal and centripetal direction (Schröder et al. 2006). This implies the existence of a protease that lies within the residual organic matrix in the spicules and that dissolves the included proteins over time. In order to test this assumption, we have perfomed zymogram analysis with extracts from M. chuni spicules. In our preparations, proteases with sizes of 130 kDa, 100 kDa, and 70 kDa have been identified. Their activities are abolished by heating the samples, thus underscoring the proteinaceous origin of the activity. In subsequent parallel studies based on size, similar proteases have also been identified in the two Demospongiae, S. domuncula and G. cydonium. At present, we hypothesize that the protease, existing within the spicules, is involved in the hydrolytic cleavage of the proteinaceous inner organic layer of the cylinder, after completion of the silica lamella.
Our analytical data, such as the presence of the major molecules known from demosponge spicules, e.g., the high-molecular-weight proteins, the 35 kDa protein representing a lectin, the proteases, and silicatein or a silicatein-like molecule, suggest a high degree of parallelism in the protein pattern and function between Demospongiae and Hexactinellida. In a final functional study, we tested whether the proteins isolated from M. chuni also displayed silicatein activity. The same assay system that had been applied previously (Cha et al. 1999; Krasko et al. 2000; Kaluzhnaya et al. 2005a, b), with the alkoxy compound TEOS, gave no indication for the presence of active silicatein molecules in M. chuni. This might indicate that no silicatein or silicatein-like proteins exist in this hexactinellid, or that such a molecule becomes functionally inactivated during the HF-dissolution process. In future studies, a less harsh dissolution procedure will be applied for the extraction of proteins from spicules of M. chuni to trace silica-synthesizing activity in this taxon.
Until now, only a working model for the synthesis of the M. chuni spicules has been proposed. Electron-microscopic studies have revealed that the tips of the spicules from this hexactinellid can be distinguished from those found in Demospongiae (Uriz et al. 2003), since all of them are open (Schulze 1904). The images show that the characteristic quadratic opening, viz., the axial canal, occasionally possesses an axial filament that reaches the upper edges of the top orifice. Taking previous data (Schulze 1904; Aizenberg et al. 2005) and the results presented here together, we consider that the spicules of Hexactinellida have been established to grow in a lamellar manner. Moreover, in view of the similarity of their structural protein pattern and their potential function, silicatein (or a silicatein-related protein) is unlikely to become associated with the surface of the spicules (Fig. 5b-1,b-2). With the longitudinal growth of the spicules, the protein mediates the silica deposition (Fig. 5b-3,b-4) and finally becomes internalized through the apical opening and associated with the axial filament (Fig. 5b-5). However, a final answer can only be given after the cloning and expression of the putative silicatein molecules in Hexactinellida.
The spicules are surrounded by collagen mats that leaves open holes with a diameter of 7–10 μm. This pattern of collagen cover around the spicules has previously been noticed by Schulze (1904) and attributed to the characteristic synthesis by the spicule-associated cells and their fixation in the mesohyl (the interior tissue with its bulky extracellular material). Until now, evidence has been lacking that these collagen fibrils are covered by silica. The morphology of the collagen fibrils in M. chuni is not common in other studied siliceous sponges (Garrone 1984). In this hexactinellid, the collagen fibrils have a pearl-string-like appearance, whereas in Demospongiae, the collagen takes the form of plain rods. The fibrils tightly surround the spicules and might be involved in the shaping of the form of the spicules. In addition, they are, as shown here, also involved in the association of the spicules among each other. This conclusion is drawn from the images of samples of tauactins and comitalia stained with Sirius Red for collagen.
To date, collagen molecules had only been described in Demospongiae (see Garrone 1998). Therefore, we screened a cDNA library from the hexactinellid A. vastus for the existence of collagen transcripts. One sequence was identified, and its deduced polypeptide was found to be highly related to the collagen alpha 1 chain precursor from H. vulgaris. The A. vastus protein comprises a 585-aa-long stretch comprising the characteristic collagen triplet repeats. As also known from other demosponge collagens, this hexactinellid-deduced protein frames the collagen triplets by non-collagen repeats.
Now that these structural and morphological data concerning the spicules of M. chuni have been obtained, the next steps will be the identification of the genes encoding the respective proteins in order to obtain the recombinant proteins and then the antibodies for light- and immuno-electron-microscopic studies. These methods should finally result in a unequivocal identification of the proteins and their functions in the synthesis of hexactinellidan spicules; such strategies have recently been successfully applied to the demosponge S. domuncula (Müller et al. 2006; Schröder et al. 2006).
We thank Ms. E. Sehn (Zoological Institute, University of Mainz, Germany) for valuable technical assistance. Material was provided by Dr. D. Bernhard (Zoological Institute, University of Leipzig, Germany), Dr. C. Lüter (Museum für Naturkunde, Berlin, Germany), Prof. Dr. R. Kinzelbach (Zoological Institute, University of Rostock, Germany), and Dr. K. Tabachnick (Institute of Oceanology, Moscow, Russia).
- Chun C (1900) Aus den Tiefen des Weltmeeres. Fischer, JenaGoogle Scholar
- Coligan JE, Dunn BM, Speicher DW, Wingfield PT (1998) Current protocols in protein science. Wiley, New York, pp 10.4.1–10.4.36Google Scholar
- Dayhoff MO, Schwartz RM, Orcutt BC (1978) A model of evolutionary change in protein. In: Dayhoff MO (ed) Atlas of protein sequence and structure. National Biomedical Research Foundation, Washington, DC, pp 345–352Google Scholar
- Ehrlich H, Hanke T, Simon P, Goebel C, Heinmann S, Born R, Worch H (2005) Demineralisation von natürlichen Silikat-basierten Biomaterialien: neue Strategie zur Isolation organischer Gerüststrukturen. Biomaterialien 6:297–302Google Scholar
- Felsenstein J (1993) PHYLIP, ver. 3.5. University of Washington, SeattleGoogle Scholar
- Garrone R (1984) Formation and involvement of extracellular matrix in the development of sponges, a primitive multicellular system. In: Trelstad RL (ed) The role of extracellular matrix in development. Liss, New York, pp 461–477Google Scholar
- Garrone R (1998) Evolution of metazoan collagens. Prog Mol Subcell Biol 21:139–191Google Scholar
- Iler RK (1979) The chemistry of silica. Wiley, New YorkGoogle Scholar
- Kennish MJ (1994) Practical handbook of marine science. CRC Press, Boca RatonGoogle Scholar
- Li J (1987) Monorhaphis intermedia—a new species of Hexactinellida. Oceanol Limnol Sinica 18:135–137Google Scholar
- Müller WEG (2005) Spatial and temporal expression patterns in animals. In: Meyers RA (ed) Encyclopedia of molecular cell biology and molecular medicine, vol 13. Wiley-VCH, Weinheim, pp 269–309Google Scholar
- Nicholas KB, Nicholas HB Jr (1997) GeneDoc: a tool for editing and annotating multiple sequence alignments. Version 1.1.004 (distributed by the author; cris.com/~ketchup/genedoc.shtml)
- Rao J, Argos P (1986) A conformational preference parameter to predict helices in integral membrane proteins. Biochem Biophys Acta 869:197–214Google Scholar
- Reitner J, Wörheide G (2002) Non-lithistid Demospongiae aps—origins of their palaeobiodiversity and highlights in history of preservation. In: Hooper JNA, Van Soest RWM (eds) Systema porifera: a guide to the classification of sponges. Kluwer Academic/Plenum, New York, pp 52–70Google Scholar
- Roux M, Bouchet P, Bourseau JP, Gaillard C, Grandperrin R, Guille A, Laurin B, Monniot C, Richer de Forges B, Rio M, Segonzac M, Vacelet J, Zibrowius H (1991) L’environment bathyal au large de la Nouvelle-Calédonie: résultats preliminaries de la campagne CALSUB et consequences paléoécologiques. Bull Soc Geol France 162:675–685Google Scholar
- Schmidt WJ (1926) Über das Wesen der Lamellierung und das gegenseitige Verhalten von organischer und anorganischer Substanz bei den Kieselschwammnadeln. Zool Jahrb (Abt Morphol) 48:311–364Google Scholar
- Schmidt WJ (1928) Die Pfahlnadel von Monorhaphis chuni F.E. Schulze. Mikrokosmos 21:113–120Google Scholar
- Schrammen A (1924) Die Kieselspongien der oberen Kreide von Nordwestdeutschland. Monogr Geol Paleontol 2:1–159Google Scholar
- Schröder HC, Perović-Ottstadt S, Rothenberger M, Wiens M, Schwertner H, Batel R, Korzhev M, Müller IM, Müller WE (2004a) Silica transport in the demosponge Suberites domuncula: fluorescence emission analysis using the PDMPO probe and cloning of a potential transporter. Biochem J 381:665–673PubMedCrossRefGoogle Scholar
- Schröder HC, Boreiko A, Korzhev M, Tahir MN, Tremel W, Eckert C, Ushijima H, Müller IM, Müller WEG (2006) Co-expression and functional interaction of silicatein with galectin: matrix-guided formation of siliceous spicules in the marine demosponge Suberites domuncula. J Biol Chem 281:12001–12009CrossRefGoogle Scholar
- Schulze FE (1904) Hexactinellida. Wissenschaftliche Ergebnisse der Deutschen Tiefsee–Expedition auf dem Dampfer “Valdivia” 1898–1899. Fischer, StuttgartGoogle Scholar
- Sommer H, Kröner A, Hauzenberger C, Muhongo S, Wingate MTD (2003) Metamorphic petrology and zircon geochronology of high-grade rocks from the central Mozambique Belt of Tanzania: crustal recycling of Archean and Palaeoproterozoic material during the Pan-African orogeny. J Metamorphic Geol 21:915–934CrossRefGoogle Scholar
- Sullivan CW (1986) Silicification in diatoms. In: Evered D, O’Connor M (eds) Silicon biochemistry. Wiley, Chichester, pp 59–89Google Scholar
- Tabachnick KR (2002) Family Monorhaphididae Ijima, 1927. In: Hooper JNA, Van Soest RWM (eds) Systema porifera. Kluwer Academic, New York, pp 1264–1266Google Scholar
- Thomas R (2000) Determination of water contents of granite melt inclusions by confocal laser Raman microprobe spectroscopy. Am Mineral 85:868–872Google Scholar
- Wang X, Wang Y (2006) An introduction to the study on natural characteristics of sponge spicules and bionic applications. Adv Earth Sci 21:37–42Google Scholar