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Cell and Tissue Research

, Volume 327, Issue 3, pp 485–492 | Cite as

Pathophysiological role of platelets in acute experimental pancreatitis: influence of endothelin A receptor blockade

  • Dirk UhlmannEmail author
  • Heike Lauer
  • Frederik Serr
  • Stefan Ludwig
  • Andrea Tannapfel
  • Martin Fiedler
  • Johann Hauss
  • Helmut Witzigmann
Regular Article

Abstract

The potential pathophysiological role of platelet-endothelium interactions was investigated during ischemia/reperfusion (I/R), and the effect of a selective endothelinA receptor antagonist (ETA-RA) was evaluated in an acute pancreatitis model. Acute pancreatitis was induced by warm ischemia (60 min) in Wistar rats, and its effects with and without antagonist treatment were investigated. Equivalent sham-operated animals were also studied. Microcirculatory changes were assessed by in vivo microscopy, and serum levels for lipase/amylase and histological specimens were investigated. Capillary constriction to 83.7 ± 6.7% of sham-operated diameters was observed after 60 min of ischemia. A capillary density of 56.8 ± 9.3% of the sham-operated group (396.3 ± 15.8 mm−1) was measured after reperfusion. Stagnant leukocytes (329.5 ± 30.4%) and platelets (337.5 ± 32.3%) increased in postcapillary venules (P < 0.05). Administration of the ETA-RA significantly reduced I/R injury. Capillary diameters were maintained (101.4 ± 4.5%), and capillary density was improved to 73.3 ± 7.6% of sham-operated animals (P < 0.05). Stagnant leukocytes (152.3 ± 10.6%) and platelets (207.1 ± 19.8%) in sinusoids and postcapillary venules were reduced (P < 0.05). The extent of acute pancreatitis was reduced in the therapy group as indicated by serum lipase/amylase values and histological tissue damage (P < 0.05). Thus, ETA-RA therapy was effective in reducing I/R-induced pancreatitis in this experimental model.

Keywords

Ischemia Reperfusion Pancreas Platelets Endothelin Rat (Wistar, female) 

Notes

Acknowledgement

We thank Knoll (Ludwigshafen, Germany) for supplying BSF 208075.

Supplementary material

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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Dirk Uhlmann
    • 1
    Email author
  • Heike Lauer
    • 1
  • Frederik Serr
    • 1
  • Stefan Ludwig
    • 1
  • Andrea Tannapfel
    • 2
  • Martin Fiedler
    • 3
  • Johann Hauss
    • 1
  • Helmut Witzigmann
    • 1
  1. 1.2nd Department of SurgeryUniversity of LeipzigLeipzigGermany
  2. 2.Institute of PathologyUniversity of LeipzigLeipzigGermany
  3. 3.Department of Laboratory Medicine, Clinical Chemistry and Molecular DiagnosticsUniversity of LeipzigLeipzigGermany

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