Cell and Tissue Research

, Volume 326, Issue 3, pp 725–736 | Cite as

Adult bone-marrow-derived mesenchymal stem cells contribute to wound healing of skin appendages

  • Haihong Li
  • Xiaobing FuEmail author
  • Yunshu Ouyang
  • Cunliang Cai
  • Jun Wang
  • Tongzhu Sun
Regular Article


Adult bone-marrow-derived mesenchymal stem cells (MSCs) are well-established as having the capacity to differentiate into cells with mesodermal, ectodermal, and endodermal characteristics and can leave their niche to home toward and engraft within foreign tissues. To investigate whether adult MSCs contribute to the repair of skin appendages after injury, BrdU-labeled MSCs were co-cultured with heat-shocked confluent sweat gland cells (SGCs) in vitro and later intravenously injected into full-thickness skin wounds in rats. When adult MSCs were co-cultured with heat-shocked SGCs, a subset of adult MSCs differentiated into SGCs, the percentage of differentiation being enhanced by epidermal growth factor and the injured microenviroment, but weakened by PD98059. The ERK (extracellular signal-regulated kinase) pathway, especially pERK, was involved in the phenotype conversion of human MSCs into human SGC. Labeled MSCs were noted in hair follicles, sebaceous glands, blood vessels, and dermis in full-thickness wounds, and the incorporated cells in hair follicles and sebaceous glands were also positive for pan-cytokeratin. After wound healing, some labeled MSCs returned to the bone marrow, whereas other were retained in the dermis. We conclude that adult MSCs have the capacity to dock at specific sites, to contribute to wound healing of skin appendages, and to home toward marrow, and that engraftment of bone-marrow-derived cells is a functional event.


Adult bone marrow mesenchymal stem cells Skin appendages Phenotype Signaling pathway Wound healing Human Rat (Wistar) 


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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Haihong Li
    • 1
    • 2
  • Xiaobing Fu
    • 1
    Email author
  • Yunshu Ouyang
    • 1
  • Cunliang Cai
    • 1
  • Jun Wang
    • 1
  • Tongzhu Sun
    • 1
  1. 1.Wound Healing and Cell Biology LaboratoryBurns Institute, The First Affiliated Hospital (304th Hospital), General Hospital of PLA, Trauma Center of Postgraduate Medical CollegeBeijingPeople’s Republic of China
  2. 2.Taihe Hospital, Yunyang Medical CollegeShiyan, HubeiPeople’s Republic of China

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