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Cell and Tissue Research

, Volume 325, Issue 3, pp 481–492 | Cite as

Appearance of cuboidal cells in relation to salinity in gills of Fundulus heteroclitus, a species exhibiting branchial Na+ but not Cl uptake in freshwater

  • Pierre LaurentEmail author
  • Claudine Chevalier
  • Chris M. Wood
Regular Article

Abstract

Fundulus heteroclitus (killifish) is a model organism for ionoregulatory studies, particularly because of its opercular epithelium, although the gills are the major sites of ion exchange. Whereas Na+ and Cl are excreted through the gills in seawater (SW), the killifish is unusual in taking up only Na+ and not Cl at the gills in freshwater (FW). We describe morphological changes in the branchial epithelium following transfer from an acclimation medium of 10% SW to 100% SW or FW. In 10% SW, mitochondria-rich cells resemble typical seawater chloride cells (SWCCs) with accessory cells. After transfer to 100% SW, no change occurs in pavement cell (PVC) morphology or mitotic rate (measured by bromo-deoxyuridine technique), although the density of SWCC apertures increases several fold because of the uncovering of buried SWCCs by PVCs, in accord with increased rates of Na+ and Cl efflux. After transfer to FW, PVC morphology remains unchanged, but SWCCs and accessory cells are quickly covered by PVCs, with many undergoing apoptosis or necrosis. The mitotic rate doubles by 10–14 h but typical freshwater chloride cells (FWCCs) do not appear. Instead, a wedge-shaped cell type that is moderately rich in apically oriented mitochondria, with a large ovoid nucleus, thin cytoplasmic layer, paucity of vesicular-tubular network, and variably villous surface rapidly (by 3 h) and progressively appears in the filament epithelium, by both uncovering and mitosis. This cell type is similar to that recently identified as the site of Na+ uptake in the FW trout gill. We propose the new term “cuboidal cell” for this cell, based on its morphology, to avoid confusion with traditional terminology (of PVC). We hypothesize that the cuboidal cells are the sites of active Na+ uptake in FW F. heteroclitus and suggest that the lack of Cl uptake is attributable to the absence of typical FWCCs previously described in teleosts.

Keywords

Cuboidal cells Chloride cells Pavement cells Gills Osmoregulation Killifish, Fundulus heteroclitus (Teleosti) 

Notes

Acknowledgements

We thank Dr. John Lott and Mr. Klaus Schultes of the Biology Electron Microscope Facility, McMaster University for assistance, and Dr. W. S. Marshall, St. Francis Xavier University, for the supply of killifish. We are also grateful to two anonymous reviewers whose constructive criticisms improved the paper.

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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Pierre Laurent
    • 1
    • 2
    Email author
  • Claudine Chevalier
    • 1
  • Chris M. Wood
    • 1
  1. 1.Department of BiologyMcMaster UniversityHamiltonCanada
  2. 2.IttenheimFrance

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