Cell and Tissue Research

, Volume 321, Issue 1, pp 75–88 | Cite as

Expression of toll-like receptor 2 and 4 in lipopolysaccharide-induced lung injury in mouse

  • Takuma Saito
  • Takashi Yamamoto
  • Toshihiro Kazawa
  • Humitake Gejyo
  • Makoto NaitoEmail author
Regular Article


Pattern recognition receptors, which include the toll-like receptors (TLRs), are considered to play an important role in the response against lipopolysaccharide (LPS). In this study, we performed a reverse transcriptase/polymerase chain reaction (RT-PCR) study, Western analysis, immunohistochemical staining, and RT-PCR-amplified in situ hybridization of TLR2 and TLR4 in the case of LPS-induced lung injury. The expression of TLR2 and TLR4 increased in the lung rapidly after LPS inhalation and peaked at 24 h, followed by a gradual decrease. TLR2 and TLR4 expression was observed on the bronchial epithelium and tissue macrophages. In the early hours after inhalation of fluorescein-isothiocyanate (FITC)-labeled LPS, LPS was detected mainly on the bronchial epithelium and on a few of tissue macrophages. One day after inhalation, the LPS signals disappeared in the lungs of the mice, except for a few alveolar macrophages. The expression of TLR2, TLR4, and CD14 was coincident with the signals of FITC-labeled LPS. Instillation of liposome-encapsulated dichloromethylene diphosphonate induced a significant decrease in alveolar macrophages. In the macrophage-depleted mice, however, expression of TLR2 and TLR4 mRNA or protein was slightly suppressed in the lung after LPS inhalation. These data suggest that the bronchial epithelium and macrophages play crucial roles in LPS-induced lung injury through TLR2 and TLR4.


Lipopolysaccharide Lung Toll-like receptor 2 Toll-like receptor 4 Mouse (ICR, female) 



We thank Mr. Susumu Momozaki and Takashi Aoyama for their excellent technical assistance. We are grateful to Kissei Pharmaceuticals for supplying dichloromethylene diphosphonate, and Nippon Fine Chemicals for providing phosphatidylcholine.


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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Takuma Saito
    • 1
    • 2
  • Takashi Yamamoto
    • 1
  • Toshihiro Kazawa
    • 1
    • 2
  • Humitake Gejyo
    • 2
  • Makoto Naito
    • 1
    Email author
  1. 1.Department of Cellular Function, Division of Cellular and Molecular PathologyNiigata University Graduate School of Medical and Dental SciencesNiigataJapan
  2. 2.Division of Respiratory MedicineNiigata University Graduate School of Medical and Dental SciencesNiigataJapan

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