Cell and Tissue Research

, Volume 324, Issue 3, pp 353–360 | Cite as

Short-chain fatty acid receptor, GPR43, is expressed by enteroendocrine cells and mucosal mast cells in rat intestine

  • Shin-ichiro Karaki
  • Retsu Mitsui
  • Hisayoshi Hayashi
  • Ikuo Kato
  • Hiroshi Sugiya
  • Toshihiko Iwanaga
  • John B. Furness
  • Atsukazu Kuwahara
Regular Article

Abstract

Short-chain fatty acids (SCFAs), such as acetate, propionate, and butyrate, are the major anions in the large intestinal lumen. They are produced from dietary fiber by bacterial fermentation and are known to have a variety of physiological and pathophysiological effects on the intestine. In the present study, we investigated the expression of the SCFA receptor, GPR43, in the rat distal ileum and colon. Expression of GPR43 was detected by reverse transcriptase/polymerase chain reaction (RT-PCR), Western blotting, and immunohistochemistry. mRNA for GPR43 was detected, by RT-PCR, in extracts of the whole wall and separated mucosa from the ileum and colon and from muscle plus submucosa from the ileum, but not from muscle plus submucosa preparations from the colon. We raised a rabbit antiserum against a synthesized fragment of rat GPR43; this was specific for rat GPR43. GPR43 protein was detected by Western blot analysis in extracts of whole wall and separated mucosa, but not in muscle plus submucosa extracts. By immunohistochemistry, GPR43 immunoreactivity was localized to enteroendocrine cells expressing peptide YY (PYY), whereas 5-hydroxytryptamine (5-HT)-immunoreactive (IR) enteroendocrine cells were not immunoreactive for GPR43. Mast cells of the lamina propria expressing 5-HT were also GPR43-IR. The results of the present study suggest that the PYY-containing enteroendocrine cells and 5-HT-containing mucosal mast cells sense SCFAs via the GPR43 receptor. This is consistent with physiological data showing that SCFAs stimulate the release of PYY and 5-HT from the ileum and colon.

Keywords

Short-chain fatty acids GPR43 Enteroendocrine cell Mucosal mast cell Peptide YY 5-Hydroxytryptamine Intestine Rat (Wistar, male) 

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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Shin-ichiro Karaki
    • 1
  • Retsu Mitsui
    • 1
  • Hisayoshi Hayashi
    • 2
  • Ikuo Kato
    • 3
  • Hiroshi Sugiya
    • 4
  • Toshihiko Iwanaga
    • 5
  • John B. Furness
    • 6
  • Atsukazu Kuwahara
    • 1
  1. 1.Laboratory of Physiology, Institute for Environmental SciencesUniversity of ShizuokaShizuokaJapan
  2. 2.Laboratory of Physiology, School of Food and Nutritional SciencesUniversity of ShizuokaShizuokaJapan
  3. 3.Yanaihara InstituteFujinomiya-shiJapan
  4. 4.Department of Physiology and Research Institute of Oral ScienceNihon University School of Dentistry at MatsudoMatsudoJapan
  5. 5.Laboratory of Histology and Cytology, Graduate School of MedicineHokkaido UniversitySapporoJapan
  6. 6.Department of Anatomy & Cell Biology and Centre for NeuroscienceUniversity of MelbourneParkvilleAustralia

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