Cell and Tissue Research

, Volume 320, Issue 1, pp 69–75 | Cite as

Insulin-like growth factor-I stimulates cell proliferation in the outer layer of Hertwig’s epithelial root sheath and elongation of the tooth root in mouse molars in vitro

  • Naoki Fujiwara
  • Makoto J. Tabata
  • Makoto Endoh
  • Kiyoto Ishizeki
  • Tokio Nawa
Regular Article


To elucidate the mechanism of root formation in tooth development, we examined the role of insulin-like growth factor I (IGF-I) on early root formation in mandibular first molar teeth from 5-day-old mice. Immunohistochemistry revealed the specific localization of the IGF-I receptor in Hertwig’s epithelial root sheath (HERS) in the tooth root. The effect of IGF-I on root development, especially on HERS, was subsequently examined in vitro. The control culture showed normal development of HERS and the periodontium, resembling that in vivo. However, the presence of 100 ng/ml IGF-I resulted in elongation of HERS and increased cell proliferation in its outer layer. These effects were negated by the addition of antibodies specific for IGF-I. Thus, we propose that IGF-I is involved in early root formation by regulating the mitotic activity in the outer layer of HERS.


Hertwig’s epithelial root sheath Insulin-like growth factor-I IGF-I receptor Organ culture Tooth root formation Mouse (ddY) 



The authors thank Dr. Yasunori Sakakura (Health Sciences University of Hokkaido) for critical reading of the manuscript.


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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Naoki Fujiwara
    • 1
  • Makoto J. Tabata
    • 2
  • Makoto Endoh
    • 1
  • Kiyoto Ishizeki
    • 1
  • Tokio Nawa
    • 1
  1. 1.Department of Oral Anatomy II, School of DentistryIwate Medical UniversityMoriokaJapan
  2. 2.Department of Anatomy for Oral Sciences, Graduate School of Medical and Dental SciencesKagoshima UniversityKagoshimaJapan

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