Cell and Tissue Research

, Volume 317, Issue 3, pp 265–277 | Cite as

Dynamics of PAF-induced conjunctivitis reveals differential expression of PAF receptor by macrophages and eosinophils in the rat

  • Olga ZinchukEmail author
  • Atsuki Fukushima
  • Eric Hangstefer
  • Hisayuki Ueno
Regular Article


The action of platelet-activating factor (PAF) toward the PAF receptor (PAF-R) plays an important role in inflammation. We employed immunohistochemistry and quantitative confocal immunofluorescence microscopy to examine the dynamic changes of PAF-R expression in the conjunctiva in response to PAF-induced conjunctivitis in Brown Norway rats within the first 24 h after topical administration of PAF. Instillation of PAF caused an alteration in pattern of recruitment of macrophages and eosinophils into the conjunctiva, as was visualized by immunohistochemical staining for the antigens ED 1 and ED 2 (markers of macrophages) and MBP (a marker of eosinophils). An increase in the number of PAF-R-positive cells was also detected. Quantitative colocalization analysis revealed the strongest rise in the degree of PAF-R expression by macrophages within the first 6 h, whereas their infiltration increased throughout the period of observation. However, eosinophils showed a high degree of PAF-R expression during all 24 h of the experiment, although they infiltrated strongly only within the first 2 h. Thus, for the first time, the use of quantitative colocalization analysis software developed by us has revealed intrinsic details of the interaction of PAF and PAF-R in conjunctivitis, findings not otherwise obtainable by using qualitative approaches alone. Our results provide a theoretical basis for a definition of the proper time-frame in which to prevent and control the infiltration of macrophages and eosinophils into the conjunctiva.


Platelet-activating factor Platelet-activating factor receptor Conjunctivitis Macrophages Eosinophils Confocal microscopy Quantitative colocalization Rat (Brown Norway) 


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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Olga Zinchuk
    • 1
    Email author
  • Atsuki Fukushima
    • 1
  • Eric Hangstefer
    • 2
  • Hisayuki Ueno
    • 1
  1. 1.Laboratory of Immunology, Department of OphthalmologyKochi Medical SchoolKochiJapan
  2. 2.Software DeveloperBoiseUSA

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