Cell and Tissue Research

, Volume 317, Issue 3, pp 319–326

Muscle satellite cell heterogeneity: in vitro and in vivo evidences for populations that fuse differently

  • Karl Rouger
  • Mickael Brault
  • Nathalie Daval
  • Isabelle Leroux
  • Lydie Guigand
  • Julie Lesoeur
  • Bernard Fernandez
  • Yan Cherel
Regular Article

DOI: 10.1007/s00441-004-0911-9

Cite this article as:
Rouger, K., Brault, M., Daval, N. et al. Cell Tissue Res (2004) 317: 319. doi:10.1007/s00441-004-0911-9

Abstract

During development, muscle growth results from the proliferation of satellite cells (SC) and their fusion with fibers. Several studies revealed heterogeneity of SC population notably based on the proliferation rate. Here, we examined the SC characteristics of turkey skeletal muscles in terms of proliferation and more specifically fusion, to define if the ability of these cells to fuse may represent a distinct characteristic between them and could be directly associated with their proliferation properties. Freshly extracted SC were plated in clonal condition and their proliferation rate was assessed 11 days later. To investigate the SC fusion behavior, in vitro and in vivo approaches were developed. Highly and slowly proliferative SC were initially labeled with a nuclear β-galactosidase (β-Gal) activity and co-cultured with differentiated primary cultures. After 5 days, distribution of β-Gal positive (β-Gal+) nuclei was examined. Also, the two labeled SC types were transplanted into different muscles in autologous model. One week later, number of β-Gal+ nuclei per fiber and diameter of fibers displaying β-Gal+ nuclei were determined. In vitro, we showed that SC from turkey skeletal muscle are present as a heterogeneous population in terms of proliferation. Examination of their fusion properties in vitro as well as in vivo revealed that highly proliferative SC exclusively exhibited fusion with differentiated myotubes or myofibers, whereas slowly proliferative SC mainly fused together. Collectively, these data demonstrate for the first time that SC with different proliferation rate also intrinsically differ in their fusion potential, suggesting distinct roles for these sub-populations in muscle growth.

Keywords

Skeletal muscle Satellite cell Cell fusion Co-culture Cell transplantation Turkey Meleagris gallopavo (Aves) 

Abbreviations

ald

Anterior latissimus dorsi

ANOVA

Analysis of variance

DMEM

Dulbecco’s modified Eagle’s medium

FCS

Fetal calf serum

HBSS

Hank’s balanced salt solution

M199

Medium 199

MGG

May-Grünwald Giemsa

MHC

Myosin heavy chain

PBS

Phosphate-buffered saline

pld

Posterior latissimus dorsi

pm

Pectoralis major

PSF

Penicillin streptomycin fungizonND

SC

Satellite cell

SD

Standard deviation

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Karl Rouger
    • 1
  • Mickael Brault
    • 1
  • Nathalie Daval
    • 1
  • Isabelle Leroux
    • 1
  • Lydie Guigand
    • 1
  • Julie Lesoeur
    • 1
  • Bernard Fernandez
    • 1
  • Yan Cherel
    • 1
  1. 1.UMR 703 INRA/Ecole Nationale VétérinaireNantesFrance

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