Differential expression of glucose-regulated protein 78 during spermatogenesis
- 226 Downloads
The aim of the present study was to isolate and identify proteins involved in the process of spermatogenesis. To achieve this goal we used the technique of proteomic analysis. Comparison of testis protein patterns obtained by high-resolution two-dimensional gel electrophoresis from 1-week- and 7-week-old mice showed significant differences in protein spot intensities. Subsequently several of these variant protein spots were identified by mass spectrometry. Glucose-regulated protein (GRP) 78 (Bip) was one of them. GRP78, expressed at a lower level in 1-week-old mouse testes compared to 7-week-old mouse testes, is a member of the heat shock 70 protein family. It has recently been shown to be important for protecting cells from apoptosis in somatic cells, especially in progressively growing tumor cells. Further, immunohistochemical (IHC) analyses of mouse and human testes sections were performed to determine the cellular distribution of this protein. A strong GRP78 staining was seen beginning with pachytene spermatocytes. These findings suggested that GRP78 might perform an important function in the process of spermatogenesis.
KeywordsGRP78 Proteomic analysis Heat shock protein 70 family Spermatogenesis Mouse (ICR) Human
We would like to thank Dr. Shwe-yan Lee (Maclehose Medical Rehabilitation Center, HongKong, China) for critically reading the manuscript, helpful suggestions, and comments.
- Lian Z, Kluger Y, Greenbaum DS, Tuck D, Gerstein M, Berliner N, Weissman SM, Newburger PE (2002) Genomic and proteomic analysis of the myeloid differentiation program: global analysis of gene expression during induced differentiation in the MPRO cell line. Blood 100:3209–3220CrossRefPubMedGoogle Scholar
- Reddy RK, Mao C, Baumeister P, Austin RC, Kaufman RJ, Lee AS (2003) Endoplasmic reticulum chaperone protein GRP78 protects cells from apoptosis induced by topoisomerase inhibitors: role of ATP binding site in suppression of caspase-7 activation. J Biol Chem 278:20915–20924CrossRefPubMedGoogle Scholar
- Russell LD, Ettlin RA, Sinha Hikim AP, Clegg ED (1990) Histological and histopathological evaluation of the testis. Cache River Press, ClearwaterGoogle Scholar
- Sha J, Zhou Z, Li J, Yin L, Yang H, Hu G, Luo M, Chan HC, Zhou K, Spermatogenesis Study Group (2002) Identification of testis development and spermatogenesis-related genes in human and mouse testes using cDNA arrays. Mol Hum Reprod 8:511–517Google Scholar
- Stern H (1993) Cell and molecular biology of the testis. In: Desjardins C, Ewing LL (eds) Oxford University Press, Oxford, pp 296–316Google Scholar
- Virve Pentikaien (2002) Regulation of male germ cell apoptosis. Helsinki University Biomedical Dissertations, No. 13. University of Helsinki, FinlandGoogle Scholar
- Yan W, Kero J, Suominen J, Toppari J (2001) Differential expression and regulation of the retinoblastoma family of proteins during testicular development and spermatogenesis: roles in the control of germ cell proliferation, differentiation and apoptosis. Oncogene 20:1343–1356CrossRefPubMedGoogle Scholar