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Cell and Tissue Research

, Volume 316, Issue 3, pp 359–367 | Cite as

Differential expression of glucose-regulated protein 78 during spermatogenesis

  • Ran Huo
  • Ye-fei Zhu
  • Xiang Ma
  • Min Lin
  • Zuo-min Zhou
  • Jia-hao ShaEmail author
Regular Article

Abstract

The aim of the present study was to isolate and identify proteins involved in the process of spermatogenesis. To achieve this goal we used the technique of proteomic analysis. Comparison of testis protein patterns obtained by high-resolution two-dimensional gel electrophoresis from 1-week- and 7-week-old mice showed significant differences in protein spot intensities. Subsequently several of these variant protein spots were identified by mass spectrometry. Glucose-regulated protein (GRP) 78 (Bip) was one of them. GRP78, expressed at a lower level in 1-week-old mouse testes compared to 7-week-old mouse testes, is a member of the heat shock 70 protein family. It has recently been shown to be important for protecting cells from apoptosis in somatic cells, especially in progressively growing tumor cells. Further, immunohistochemical (IHC) analyses of mouse and human testes sections were performed to determine the cellular distribution of this protein. A strong GRP78 staining was seen beginning with pachytene spermatocytes. These findings suggested that GRP78 might perform an important function in the process of spermatogenesis.

Keywords

GRP78 Proteomic analysis Heat shock protein 70 family Spermatogenesis Mouse (ICR) Human 

Notes

Acknowledgements

We would like to thank Dr. Shwe-yan Lee (Maclehose Medical Rehabilitation Center, HongKong, China) for critically reading the manuscript, helpful suggestions, and comments.

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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Ran Huo
    • 1
  • Ye-fei Zhu
    • 1
  • Xiang Ma
    • 1
  • Min Lin
    • 1
  • Zuo-min Zhou
    • 1
  • Jia-hao Sha
    • 1
    Email author
  1. 1.Laboratory of Reproductive MedicineNanjing Medical UniversityNanjingChina

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