Custom-shaping system for bone regeneration by seeding marrow stromal cells onto a web-like biodegradable hybrid sheet
- 129 Downloads
New bone for the repair or the restoration of the function of traumatized, damaged, or lost bone is a major clinical need, and bone tissue engineering has been heralded as an alternative strategy for regenerating bone. A novel web-like structured biodegradable hybrid sheet has been developed for bone tissue engineering by preparing knitted poly(DL-lactic-co-glycolic acid) sheets (PLGA sheets) with collagen microsponges in their openings. The PLGA skeleton facilitates the formation of the hybrid sheets into desired shapes, and the collagen microsponges in the pores of the PLGA sheet promote cell adhesion and uniform cell distribution throughout the sheet. A large number of osteoblasts established from marrow stroma adhere to the scaffolds and generate the desired-shaped bone in combination with these novel sheets. These results indicate that the web-like structured novel sheet shows promise for use as a tool for custom-shaped bone regeneration in basic research on osteogenesis and for the development of therapeutic applications.
KeywordsBone regeneration Tissue engineering Scaffold Marrow stroma Polymer KUSA-A1 cells
We sincerely thank Y. Takeda and S. Matsumoto for support throughout the work, and N. Hida, T. Inomata, Y. Hashimoto, and Y. Nakamura for providing expert technical assistance.
- Gopferich A, Peter SJ, Lucke A, Lu L, Mikos AG (1999) Modulation of marrow stromal cell function using poly(D,L-lactic acid)-block-poly(ethylene glycol)-monomethyl ether surfaces J Biomed Mater Res 46:390–398Google Scholar
- Kohyama J, Abe H, Shimazaki T, Koizumi A, Nakashima K, Gojo S, Taga T, Okano H, Hata J, Umezawa A (2001) Brain from bone: efficient meta-differentiation of marrow stroma-derived mature osteoblasts to neurons with Noggin or a demethylating agent. Differentiation 68:235–244CrossRefPubMedGoogle Scholar
- Laurencin CT, El-Amin SF, Ibim SE, Willoughby DA, Attawia M, Allcock HR, Ambrosio AA (1996) A highly porous 3-dimensional polyphosphazene polymer matrix for skeletal tissue regeneration J Biomed Mater Res 30:133–138Google Scholar
- Ochi K, Chen G, Ushida T, Gojo S, Segawa K, Tai H, Ueno K, Ohkawa H, Mori T, Yamaguchi A, Toyama Y, Hata J, Umezawa A (2003) Use of isolated mature osteoblasts in abundance acts as desired-shaped bone regeneration in combination with a modified poly-DL-lactic-co-glycolic acid (PLGA)-collagen sponge. J Cell Physiol 194:45–53CrossRefPubMedGoogle Scholar
- Peter SJ, Lu L, Kim DJ, Stamatas GN, Miller MJ, Yaszemski MJ, Mikos AG (2000) Effects of transforming growth factor beta1 released from biodegradable polymer microparticles on marrow stromal osteoblasts cultured on poly(propylene fumarate) substrates. J Biomed Mater Res 50:452–462CrossRefPubMedGoogle Scholar
- Pitaru S, Kotev-Emeth S, Noff D, Kaffuler S, Savion N (1993) Effect of basic fibroblast growth factor on the growth and differentiation of adult stromal bone marrow cells: enhanced development of mineralized bone-like tissue in culture. J Bone Miner Res 8:919–929Google Scholar
- Sato T, Chen G, Ushida T, Ishii T, Ochiai N, Tateishi T (2001) Tissue-engineered cartilage by in vivo culturing of chondrocytes in PLGA-collagen hybrid sponge. Mater Sci Eng C17:83–89Google Scholar
- Umezawa A, Tachibana K, Harigaya K, Kusakari S, Kato S, Watanabe Y, Takano T (1991) Colony-stimulating factor 1 expression is down-regulated during the adipocyte differentiation of H-1/A marrow stromal cells and induced by cachectin/tumor necrosis factor. Mol Cell Biol 11:920–927PubMedGoogle Scholar