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Cell and Tissue Research

, Volume 314, Issue 2, pp 215–221 | Cite as

Immunobiology of chicken germinal center: II. Accumulation of apoptotic cells within the germinal center

  • Masahiro Yasuda
  • Hiroyuki Horiuch
  • Haruo Matsuda
  • Shuichi FurusawaEmail author
Regular Article

Abstract.

The germinal center (GC) develops after antigen stimulation and is thought to occur at the site of various immune responses. We observed apoptotic cells within the GC using in situ end labeling (TUNEL), small amount DNA ladder assay, and RT-PCR analysis of Bcl-2 mRNA expression. Apoptosis was detected within GCs at all phases of the GC reaction by both TUNEL and DNA ladder assays. The number of TUNEL+ nuclei within the GC did not increase over the course of the GC reaction. However, the density of DNA in the ladder assay was higher in later-phase GCs. Bcl-2 mRNA expression was detected within GCs during the early phases of the GC reaction. These results indicate that accumulation of apoptotic cells and rescue from apoptosis occur within chicken GCs. In the present paper, the reasons for the accumulation of apoptotic cells will be discussed.

Keywords

Germinal center Apoptosis Hapten Bcl-2 Chicken (White Leghorn) 

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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • Masahiro Yasuda
    • 1
    • 3
  • Hiroyuki Horiuch
    • 1
  • Haruo Matsuda
    • 1
  • Shuichi Furusawa
    • 1
    • 2
    Email author
  1. 1.Laboratory of Immunobiology, Department of Molecular and Applied Bioscience, Graduate School of Biosphere ScienceHiroshima UniversityHiroshimaJapan
  2. 2.Laboratory of Immunobiology, Department of Molecular and Applied Bioscience, Graduate School of Biosphere ScienceHiroshima UniversityHiroshimaJapan
  3. 3.Department of Veterinary Anatomy, Faculty of AgricultureMiyazaki UniversityMiyazakiJapan

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