Cell and Tissue Research

, Volume 314, Issue 2, pp 237–249 | Cite as

Role of tumor metastasis suppressor gene KAI1 in digestive tract carcinomas and cancer cells

  • Qiao Wu
  • Yuan Ji
  • Ming-qing Zhang
  • Yu-qiang Chen
  • Fu Chen
  • Da-lin Shi
  • Zong-hui Zheng
  • Yao-jian Huang
  • Wen-jin Su
Regular Article

Abstract

The KAI1 gene is identified as a tumor metastasis suppressor gene in many types of cancer. We examined KAI1 gene and its protein KAI1/CD82 expression by in situ hybridization and immunohistochemical analysis, and found that KAI1 mRNA and protein expression were inversely correlated with lymph node and distant metastasis in digestive tract carcinomas, but not with age and gender of the patient, or with tumor differentiation. Moreover, KAI1/CD82 protein expression positively reflected the survival outcome of patients. Western blot analysis showed that VP-16 increased KAI1/CD82 protein expression obviously in various cancer cell lines, especially in those that were highly metastatic. This increased KAI1/CD82 expression was associated with its translocation from the cytomembrane to the nucleus, in which it interacted with nuclear p53 protein, forming a strong complex, observed by confocal microscopy and co-immunoprecipitation, respectively. In nude mice, after feeding with VP-16, the number of tumors metastasized from spleen to liver was obviously reduced, and KAI1/CD82 protein expression became stronger in those metastatic tumors. Accordingly, this demonstrated that KAI1 might be used as an indicator for predicting the clinical outcome, and VP-16 may be clinically considered as a promising candidate for anti-metastasis with regard to its potential to upregulate KAI1 expression.

Keywords

KAI1 p53 VP-16 Digestive tract carcinoma Human 

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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • Qiao Wu
    • 1
  • Yuan Ji
    • 1
  • Ming-qing Zhang
    • 1
    • 2
  • Yu-qiang Chen
    • 1
    • 1
    • 3
  • Fu Chen
    • 1
  • Da-lin Shi
    • 1
  • Zong-hui Zheng
    • 1
  • Yao-jian Huang
    • 1
  • Wen-jin Su
    • 1
  1. 1.Key Laboratory of the Ministry of Education for Cell Biology and Tumor Cell Engineering, School of Life SciencesXiamen UniversityXiamenChina
  2. 2.Dong Nan HospitalChina
  3. 3.174 HospitalChina

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