Cell and Tissue Research

, Volume 313, Issue 3, pp 301–311 | Cite as

Endoreplication and polyploidy in primary culture of rat hepatic stellate cells

  • Jozsef Dudas
  • Bernhard Saile
  • Hamudi El-Armouche
  • Isabella Aprigliano
  • Giuliano Ramadori
Regular Article

Abstract

Hepatic stellate cells (HSCs), the pericytes of hepatic sinusoids, and liver myofibroblasts (rMFs), cells located in the portal field and around the pericentral area, are the principal fibrogenic cell types of the liver. In cases of liver damage HSCs undergo "activation," i.e., they acquire a myofibroblast-like appearance and synthesize huge amounts of extracellular matrix proteins (ECMs). Their proliferation ability, however, is a matter of debate. In fact, during culture the number of rat HSCs decreases, while DNA synthesis activity and DNA content per cell increase (4±0.6 times). Together with the decrease in cell number (60±19% at day 6 of primary culture compared to day 3), cell volume increases and many HSCs become multinuclear. On the other hand, in cultures of rMFs, cell number increases along with DNA synthesis, and these cells do not become multinuclear. "Activated" HSCs produce higher levels of cyclin D1 and E1 transcripts than rMFs, which correlates with their increased levels of phosphorylated retinoblastoma (Rb) protein. In activated HSCs DNA synthesis seems to be associated with polyploidy and increase in cell volume, while DNA synthesis is followed by mitosis in rMFs.

Keywords

HSC Endoreplication Myofibroblast pRb Cyclin D1 Rat (Wistar) 

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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • Jozsef Dudas
    • 1
  • Bernhard Saile
    • 1
  • Hamudi El-Armouche
    • 1
  • Isabella Aprigliano
    • 1
  • Giuliano Ramadori
    • 1
  1. 1.Department of Internal Medicine, Section of Gastroenterology and EndocrinologyGeorg-August University GöttingenGöttingenGermany

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