Cell and Tissue Research

, Volume 311, Issue 1, pp 47–51

Evidence that exogenous substances can be phagocytized by alveolar epithelial cells and transported into blood capillaries

  • Tomoko Kato
  • Takashi Yashiro
  • Yoshio Murata
  • Damon C. Herbert
  • Katsuhisa Oshikawa
  • Masashi Bando
  • Shoji Ohno
  • Yukihiko Sugiyama
Regular Article

DOI: 10.1007/s00441-002-0647-3

Cite this article as:
Kato, T., Yashiro, T., Murata, Y. et al. Cell Tissue Res (2003) 311: 47. doi:10.1007/s00441-002-0647-3

Abstract.

Since the ability of alveolar epithelial cells to ingest inhaled fine particles has not been characterized in detail, the present study seeks to evaluate this physiological activity. We used a 0.2% suspension of intact or lecithin-coated polystyrene latex beads (240 nm in diameter). A 5-ml suspension of intact or lecithin-coated latex beads was intratracheally administered to rats using a compressor nebulizer. Thereafter, the lungs were perfused intratracheally with glutaraldehyde solution and cut into small pieces. The samples were postfixed with osmium tetroxide, embedded in epoxy resin and examined under an electron microscope. Both lecithin-coated and uncoated beads were incorporated into alveolar macrophages. Some of the ingested beads in the alveolar macrophages were sequestered within lysosomes. Types I and II alveolar epithelial cells selectively incorporated only lecithin-coated beads, which were also observed within the cytoplasm of monocytes in the capillary lumen. These findings suggest that alveolar epithelial cells can incorporate exogenous particles, which are then transferred from the alveoli to intravascular spaces by transcytosis.

Alveolar macrophage Polystyrene latex beads Lecithin Uptake Rat (Sprague Dawley) 

Copyright information

© Springer-Verlag 2002

Authors and Affiliations

  • Tomoko Kato
    • 1
  • Takashi Yashiro
    • 2
  • Yoshio Murata
    • 3
  • Damon C. Herbert
    • 4
  • Katsuhisa Oshikawa
    • 1
  • Masashi Bando
    • 1
  • Shoji Ohno
    • 1
  • Yukihiko Sugiyama
    • 1
  1. 1.Division of Pulmonary Medicine, Department of Medicine, Jichi Medical School, 3311–1 Yakushiji, Minamikawachi-machi, Kawachi-gun, Tochigi, 329–0498, Japan
  2. 2.Division of Histology and Cell Biology, Department of Anatomy, Jichi Medical School, 3311–1 Yakushiji, Minamikawachi-machi, Kawachi-gun, Tochigi, 329–0498, Japan
  3. 3.Department of Chemistry, Faculty of Science, Fukuoka University, 8–19–1 Nanakuma, Jonan-ku, Fukuoka 814–0108, Japan
  4. 4.Department of Cellular and Structural Biology, The University of Texas Health Science Center at San Antonio, San Antonio, TX 78229–3900, USA

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