Cell and Tissue Research

, Volume 310, Issue 2, pp 169–175

Cellular distribution and contribution of cyclooxygenase (COX)-2 to diabetogenesis in NOD mouse

  • Cheng Luo
  • Markku Kallajoki
  • Rene Gross
  • Mika Mulari
  • Tamara Teros
  • Laura Ylinen
  • Marjaana Mäkinen
  • Jukka Laine
  • Olli Simell
Regular Article

DOI: 10.1007/s00441-002-0628-6

Cite this article as:
Luo, C., Kallajoki, M., Gross, R. et al. Cell Tissue Res (2002) 310: 169. doi:10.1007/s00441-002-0628-6

Abstract.

Unlike most other mammalian cells, β-cells of Langerhans constitutively express cyclooxygenase (COX)-2 rather than COX-1. COX-2 is also constitutively expressed in type 1 diabetes (T1D) patients' periphery blood monocytes and macrophage. To understand the role of COX-2 in the β-cell, we investigated COX-2 expression in β-cells and islet infiltrates of NOD and BALB/c mice using fluorescence immunohistochemistry and cytochemical confocal microscopy and Western blotting. Immunostaining showed that COX-2 is expressed in islet-infiltrating macrophages, and that the expression of insulin and COX-2 disappeared concomitantly from the β-cells when NOD mice progressed toward overt diabetes. Also cultured INS-1E cells coexpressed insulin and COX-2 but clearly in different subcellular compartments. Treatment with celecoxib increased insulin release from these cells in a dose-dependent manner in glucose concentrations ranging from 5 to 17 mM. Excessive COX-2 expression by the islet-infiltrating macrophages may contribute to the β-cell death during insulitis. The effects of celecoxib on INS-1E cells suggest that PGE2 and other downstream products of COX-2 may contribute to the regulation of insulin release from the β-cells.

COX-2 Macrophage Confocal microscopy INS-1 cells Non obese diabetic (NOD) mouse Mouse (BALB/c) 

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Copyright information

© Springer-Verlag 2002

Authors and Affiliations

  • Cheng Luo
    • 1
  • Markku Kallajoki
    • 4
  • Rene Gross
    • 6
  • Mika Mulari
    • 5
  • Tamara Teros
    • 1
  • Laura Ylinen
    • 1
  • Marjaana Mäkinen
    • 1
  • Jukka Laine
    • 4
  • Olli Simell
    • 1
  1. 1.Juvenile Diabetes Research Foundation (FDRF) Center for Prevention of Type 1 Diabetes in Finland, University of Turku, Turku, Finland
  2. 2.MediCity Research Laboratory, University of Turku, Turku, Finland
  3. 3.Department of Pediatrics, University of Turku, Turku, Finland
  4. 4.Department of Pathology, University of Turku, Turku, Finland
  5. 5.Department of Anatomy, University of Turku, Turku, Finland
  6. 6.UMR 5094 du Centre National de la Recherche Scientifique (CNRS), Université Montpellier I, Montpellier, France
  7. 7.MediCity Research Laboratory, University of Turku, Tykistökatu 6 A, 20520, Turku, Finland

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