Cell and Tissue Research

, Volume 307, Issue 2, pp 237–242

The glucose transport facilitator GLUT8 is predominantly associated with the acrosomal region of mature spermatozoa

  • Annette Schürmann
  • Hubertus Axer
  • Andrea Scheepers
  • Holger Doege
  • Hans-Georg Joost
Regular Article

Abstract.

The glucose transporter 8 (GLUT8) is a recently identified member of the family of sugar transport facilitators. In human tissues GLUT8 is predominantly expressed in testis in a gonadotropin-dependent manner. It is shown here that the onset of mRNA synthesis of GLUT8 during the maturation of mouse testis coincides with the appearance of mature spermatozoa. Furthermore, immunohistochemistry with antiserum against the C-terminus of GLUT8 indicated that the protein was associated with spermatozoa within the seminiferous and the epididymal tubules. The GLUT8 immunoreactivity was detected within the head of mouse and human spermatozoa in the acrosomal region, and appeared to be located at the plasma membrane as well as within the cells. This specific expression and localization of GLUT8 suggests that the transport facilitator plays a major role in the fuel supply of mature spermatozoa, and that it is a potential target for inhibition of sperm cell function.

Glucose transport Spermatozoa Sugar transport facilitators Acrosome Immunocytochemistry Mouse Human 

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Copyright information

© Springer-Verlag 2002

Authors and Affiliations

  • Annette Schürmann
    • 1
  • Hubertus Axer
    • 2
  • Andrea Scheepers
    • 1
  • Holger Doege
    • 1
  • Hans-Georg Joost
    • 1
  1. 1.Institute of Pharmacology and Toxicology, Medical Faculty, Technical University of Aachen, Aachen, GermanyGermany
  2. 2.Institute of Anatomy, Medical Faculty, Technical University of Aachen, GermanyGermany
  3. 3.Institut für Pharmakologie und Toxikologie, Medizinische Fakultät der RWTH Aachen, Wendlingweg 2, 52057 Aachen, GermanyGermany

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