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Human Genetics

, Volume 101, Issue 2, pp 121–125 | Cite as

An apoptosis-inducing genotoxin differentiates heterozygotic carriers for Werner helicase mutations from wild-type and homozygous mutants

  • Charles E. Ogburn
  • Junko Oshima
  • Martin Poot
  • R. Chen
  • Kristin E. Hunt
  • Katherine A. Gollahon
  • Peter S. Rabinovitch
  • G. M. Martin
Original investigation

Abstract

Immortalized B lymphocytes from Werner syndrome subjects are shown to be hypersensitive to 4-nitroquinoline-1-oxide (4NQO), supporting earlier work on T lymphocytes. We also show that B cell lines from clinically normal heterozygous carriers exhibit sensitivities to this genotoxic agent, which are intermediate to those of wild-type and homozygous mutants. 4NQO is shown to induce an apoptotic response. These data encourage research on DNA repair with such cell lines and raise the question of an enhanced sensitivity of the relatively prevalent heterozygous carriers to certain environmental genotoxic agents.

Keywords

Homozygous Mutant Apoptotic Response Heterozygous Carrier Genotoxic Agent Werner Syndrome 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • Charles E. Ogburn
    • 1
  • Junko Oshima
    • 1
  • Martin Poot
    • 1
  • R. Chen
    • 1
  • Kristin E. Hunt
    • 1
  • Katherine A. Gollahon
    • 1
  • Peter S. Rabinovitch
    • 1
  • G. M. Martin
    • 1
  1. 1.Department of Pathology, Box 357470, 1959 N.E. Pacific Avenue, Health Sciences Building, Seattle, WA 98195, USA Tel.: +1-206-543-5088; Fax: +1-206-685-8356; e-mail: gmmartin@u.washington.eduUS

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