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Human Genetics

, Volume 99, Issue 3, pp 329–333 | Cite as

Isolation of a cDNA encoding the human homolog of COX17, a yeast gene essential for mitochondrial copper recruitment

  • Ravi Amaravadi
  • D. Moira Glerum
  • A. Tzagoloff
Original investigation

Abstract

The COX17 gene of Saccharomyces cerevisiae codes for a cytoplasmic protein essential for the expression of functional cytochrome oxidase. This protein has been implicated in targeting copper to mitochondria. To determine if Cox17p is present in mammalian cells, a yeast strain carrying a null mutation in COX17 was transformed with a human cDNA expression library. All the respiratory competent clones obtained from the transformations carried a common cDNA sequence with a reading frame predicting a product homologous to yeast Cox17p. The cloning of a mammalian COX17 homolog suggests that the encoded product is likely to function in copper recruitment in eucaryotic cells in general. Its presence in humans provides a possible target for genetically inherited deficiencies in cytochrome oxidase.

Keywords

Saccharomyces Cerevisiae Cytochrome Oxidase Null Mutation Human Homolog COX17 Gene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • Ravi Amaravadi
    • 1
  • D. Moira Glerum
    • 1
  • A. Tzagoloff
    • 1
  1. 1.Department of Biological Sciences, Columbia University, New York, NY 10027, USA Tel.: +1-212-854-2920; Fax: +1-212-865-8246US

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