Human Genetics

, Volume 98, Issue 2, pp 162–166

Fetal cells in maternal blood: recovery by charge flow separation

  • S. S. Wachtel
  • David Sammons
  • Michael Manley
  • Gwendolyn Wachtel
  • Garland Twitty
  • Joseph Utermohlen
  • Owen P. Phillips
  • Lee P. Shulman
  • Douglas J. Taron
  • U. R. Müller
  • Peter Koeppen
  • Teresa M. Ruffalo
  • Karen Addis
  • Richard Porreco
  • Joyce Murata-Collins
  • Natalie B. Parker
  • Loris McGavran
Original investigation

Abstract

Fetal blood cells can be recovered from the maternal circulation by charge flow separation (CFS), a method that obviates the risks associated with amniocentesis and chorionic villus sampling. By CFS, we processed blood samples from 13 women carrying male fetuses, 2 carrying fetuses with trisomy 21, and 1 who had delivered a stillborn infant with trisomy 18. On average more than 2000 fetal nucleated red blood cells were recovered per 20-ml sample of maternal blood. Recovery of fetal cells was confirmed by fluorescence in situ hybridization with probes for chromosomes Y, 18 and 21. After culturing of CFS-processed cells, amplification by the polymerase chain reaction revealed Y-chromosomal DNA in clones from four of six women bearing male fetuses, but not in clones from three women bearing female fetuses.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1996

Authors and Affiliations

  • S. S. Wachtel
    • 1
  • David Sammons
    • 2
  • Michael Manley
    • 2
  • Gwendolyn Wachtel
    • 1
  • Garland Twitty
    • 2
  • Joseph Utermohlen
    • 2
  • Owen P. Phillips
    • 1
  • Lee P. Shulman
    • 1
  • Douglas J. Taron
    • 3
  • U. R. Müller
    • 3
  • Peter Koeppen
    • 3
  • Teresa M. Ruffalo
    • 3
  • Karen Addis
    • 4
  • Richard Porreco
    • 5
  • Joyce Murata-Collins
    • 6
  • Natalie B. Parker
    • 6
  • Loris McGavran
    • 6
  1. 1.Department of Obstetrics and Gynecology, University of Tennessee, Memphis TN 38103, USAUS
  2. 2.BioSeparations Inc., Tucson AZ 85719, USAUS
  3. 3.Vysis Inc., Downers Grove IL 60515, USAUS
  4. 4.Copperstate Ob-Gyn, Tucson AZ 85712, USAUS
  5. 5.Rocky Mountain Perinatal Associates, Denver CO 80218, USAUS
  6. 6.The Children’s Hospital and The University of Colorado School of Medicine, Denver CO 80218, USAUS

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