Human Genetics

, Volume 132, Issue 12, pp 1405–1411 | Cite as

HLA-DPB1*04:01 allele is associated with non-obstructive azoospermia in Japanese patients

  • Timothy A. Jinam
  • Hirofumi Nakaoka
  • Kazuyoshi Hosomichi
  • Shigeki Mitsunaga
  • Hiroyuki Okada
  • Atsushi Tanaka
  • Kenichi Tanaka
  • Ituro InoueEmail author
Original Investigation


Azoospermia is defined by absence of sperm in the semen and can either be caused by obstruction of the seminal tract (obstructive azoospermia) or by defects in spermatogenesis (non-obstructive azoospermia, NOA). Previous studies reported that specific alleles and single nucleotide polymorphisms (SNPs) in the human leukocyte antigen (HLA) region were associated with NOA in East Asians. We attempt to expand upon previous findings by genotyping more HLA genes and to replicate SNP associations by focusing on Japanese NOA patients. HLA typing of six genes (HLA-A, -B, -C, -DRB1, -DQB1, and -DPB1) was done on 355 NOA patients using SSO-Luminex assay while genotyping of two previously reported SNPs (rs498422 and rs3129878) was done on 443 patients and 544 fertile males using TaqMan assay. Association between the HLA alleles and SNP with NOA was assessed with Chi squared and logistic regression tests. We found that HLA-DPB1*04:01 [corrected p value, P c 7.13 × 10−6; odds ratio (OR) 2.52], DRB1*13:02 (P c 4.93 × 10−4, OR 1.97), DQB1*06:04 (P c 8.94 × 10−4, OR 1.91) and rs3129878 (p value 3.98 × 10−4; OR 1.32) showed significant association with NOA, however, these loci are in linkage disequilibrium with each other. The conditional logistic regression tests showed that DPB1*04:01 is independently associated with NOA, confirming the involvement of the HLA region in the etiology of NOA in Japanese patients.


Human Leukocyte Antigen Varicocele Human Leukocyte Antigen Class Male Infertility Conditional Logistic Regression 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



The authors would like to thank to Ms. Satoh (NIG) and Ms. Okudaira (Tokai University) for their technical assistances. This work was supported by the Grant-in-Aid for Scientific Research on Innovative Areas (22133002), and “Genome Science” (No.221S0002) from Ministry of Education, Culture, Sports, Science and Technology of Japan (K.H, I.I).

Supplementary material

439_2013_1347_MOESM1_ESM.pdf (131 kb)
Supplementary material (PDF 130 kb)


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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Timothy A. Jinam
    • 1
  • Hirofumi Nakaoka
    • 1
  • Kazuyoshi Hosomichi
    • 1
  • Shigeki Mitsunaga
    • 2
  • Hiroyuki Okada
    • 3
  • Atsushi Tanaka
    • 4
  • Kenichi Tanaka
    • 5
  • Ituro Inoue
    • 1
    Email author
  1. 1.Division of Human GeneticsNational Institute of GeneticsMishimaJapan
  2. 2.Department of Molecular Life SciencesTokai University School of MedicineIseharaJapan
  3. 3.Department of Obstetrics and GynaecologyJyoetsu General HospitalJyoetsuJapan
  4. 4.St. Mother’s HospitalKitakyushuJapan
  5. 5.Department of Obstetrics and GynaecologyNiigata University Graduate School of Medical and Dental SciencesNiigataJapan

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