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Molecular Genetics and Genomics

, Volume 265, Issue 4, pp 694–704 | Cite as

Analysis of the introgression of Solanum bulbocastanum DNA into potato breeding lines

  •  S. Naess
  •  J. Bradeen
  •  S. Wielgus
  •  G. Haberlach
  •  J. McGrath
  •  J. Helgeson
Original Paper

Abstract.

Somatic hybrids have been obtained between potato and Solanum bulbocastanum PI 245310, a Mexican diploid (2n=2x=24) species. Through restriction fragment length polymorphism (RFLP) and randomly amplified polymorphic DNA (RAPD) analyses it was found that the somatic hybrids contain each chromosome of the diploid parent and that the synteny of RFLP markers noted with tomato, potato and S. brevidens is largely maintained in S. bulbocastanum. RFLP analyses of BC1 progeny of two different hybrids indicated that a substantial number of markers were either lost or were heterozygous, in marked contrast with results previously noted with S. brevidens. A RAPD map for all 12 chromosomes of S. bulbocastanum was prepared and marker transmission was followed in three BC2 populations. Results with chromosomes 3, 8 and 10 from these populations are compared.

Somatic hybrid potato Solanum bulbocastanum RFLP RAPD 

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Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  •  S. Naess
    • 1
  •  J. Bradeen
    • 1
  •  S. Wielgus
    • 1
  •  G. Haberlach
    • 1
  •  J. McGrath
    • 1
  •  J. Helgeson
    • 1
  1. 1.USDA/ARS Plant Disease Resistance Research Unit, Department of Plant Pathology, University of Wisconsin, Madison, WI 53706, USA

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