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Molecular and General Genetics MGG

, Volume 262, Issue 3, pp 453–461 | Cite as

Isolation and functional characterisation of a novel type of carotenoid biosynthetic gene from Xanthophyllomyces dendrorhous

  • J. C. Verdoes
  • P. Krubasik
  • G. Sandmann
  • A. J. J. van Ooyen
ORIGINAL PAPER

Abstract

The red heterobasidiomycetous yeast Xanthophyllomyces dendrorhous (perfect state of Phaffia rhodozyma) contains a novel type of carotenoid biosynthetic enzyme. Its structural gene, designated crtYB, was isolated by functional complementation in a genetically modified, carotenogenic Escherichia coli strain. Expression studies in different carotenogenic E. coli strains demonstrated that the crtYB gene encodes a bifunctional protein involved both in synthesis of phytoene from geranylgeranyl diphosphate and in cyclisation of lycopene to β-carotene. By sequence comparison with other phytoene synthases and complementation studies in E. coli with various deletion derivatives of the crtYB gene, the regions responsible for phytoene synthesis and lycopene cyclisation were localised within the protein.

Key words Phytoene synthase Lycopene cyclase Complementation Astaxanthin Evolution 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • J. C. Verdoes
    • 1
  • P. Krubasik
    • 2
  • G. Sandmann
    • 2
  • A. J. J. van Ooyen
    • 1
  1. 1.Division of Industrial Microbiology, Department of Food Technology and Nutritional Sciences, Wageningen University, P.O. Box 8129, 6700 EV Wageningen, The Netherlands E-mail: Jan.Verdoes@imb.ftns.wau.nl Tel.: +31-317-482302; Fax: +31-317-484978NL
  2. 2.Botanisches Institut, Fachbereich Biologie, J.W. Goethe Universität, PO Box 111932, D-60054 Frankfurt am Main, GermanyDE

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