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Molecular and General Genetics MGG

, Volume 260, Issue 1, pp 92–101 | Cite as

Chromosome landing at the barley Rar1 locus

  • T. Lahaye
  • K. Shirasu
  • P. Schulze-Lefert
ORIGINAL PAPER

Abstract

The barley Rar1 gene is an essential component of the race-specific, Mla-12-specified powdery mildew resistance reaction. As part of a map-based cloning strategy designed to isolate Rar1, five barley yeast artificial chromosomes (YACs) have been identified, ranging in size from 300 to 1100 kb. PCR-based YAC end-specific markers have been established and were employed to construct a local YAC contig. Four out of five YAC clones were found to be non-colinear with the source DNA. High-resolution genetic mapping of the YAC ends demonstrated that the set of five overlapping YAC clones encompasses the barley Rar1 gene.

Key words Resistance reaction Powdery mildew Yeast artificial chromosome (YAC) Map-based cloning Cleaved amplified polymorphic sequence (CAPS) 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • T. Lahaye
    • 1
  • K. Shirasu
    • 1
  • P. Schulze-Lefert
    • 1
  1. 1.The Sainsbury Laboratory, John Innes Centre Norwich Research Park, Colney Lane Norwich NR4 7UH, UK Fax: +44-1603-250024; e-mail: schlef@bbsrc.ac.ukGB

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