Molecular and General Genetics MGG

, Volume 259, Issue 3, pp 233–245

Isolation, characterisation and mapping of simple sequence repeat loci in potato

  • D. Milbourne
  • R. C. Meyer
  • A. J. Collins
  • L. D. Ramsay
  • C. Gebhardt
  • R. Waugh
ORIGINAL PAPER

DOI: 10.1007/s004380050809

Cite this article as:
Milbourne, D., Meyer, R., Collins, A. et al. Mol Gen Genet (1998) 259: 233. doi:10.1007/s004380050809

Abstract

Solanum tuberosum L. DNA sequences containing simple sequence repeat (SSR) motifs were extracted from the EMBL database, cDNA and selectively enriched small-insert DNA libraries. Enrichment was achieved using either triplex affinity capture or single-strand hybridisation selection. One hundred and twelve primer pairs which successfully amplified products of the correct size from potato DNA were ultimately designed and synthesised. Ninety-eight of these revealed length polymorphisms in a panel of four diploid and two tetraploid clones, in agreement with the high information content of this class of markers which has been found in other species. All of the markers were assigned a quality score of 1–5 based on their potential usefulness. Eighty-nine loci from 65 of the primer pairs were located on two genetic linkage maps of potato by segregation analysis of the amplified alleles. Fifty-two of the SSRs were clearly single locus. The maps were aligned using 23 SSR primer pairs and 13 RFLP loci mapped in both populations. The markers described constitute a class which should replace Restriction Fragment Length Polymorphisms (RFLP) as the markers of choice for future genetic studies in potato. The sequences of the primers, together with other information on these markers are provided.

Key words Simple sequence repeat Linkage map Single-strand hybridisation Triplex affinity capture Enriched library 

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • D. Milbourne
    • 1
  • R. C. Meyer
    • 1
  • A. J. Collins
    • 1
  • L. D. Ramsay
    • 1
  • C. Gebhardt
    • 3
  • R. Waugh
    • 2
  1. 1.Department of Cell and Molecular Genetics, Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, Scotland, UK GB
  2. 2.Department of Crop Genetics, Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, Scotland, UK Fax: 01382 562426; e-mail: rwaugh@scri.sari.ac.ukGB
  3. 3.Max-Planck-Institut für Züchtungforschung, Carl-von-Linné-Weg 10, D-50829, Köln, GermanyDE

Personalised recommendations