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Molecular and General Genetics MGG

, Volume 259, Issue 1, pp 54–59 | Cite as

Induction by alkaloids and phenobarbital of Family 4 Cytochrome P450s in Drosophila : evidence for involvement in host plant utilization

  • P. B. Danielson
  • J. L. M. Foster
  • M. M. McMahill
  • M. K. Smith
  • J. C. Fogleman
ORIGINAL PAPER

Abstract

In vertebrates, cytochrome P450s of the CYP2 and CYP3 families play a dominant role in drug metabolism, while in insects members of the CYP6 and CYP28 families have been implicated in metabolism of insecticides and toxic natural plant compounds. A degenerate 3 RACE strategy resulted in the identification of fifteen novel P450s from an alkaloid-resistant species of Drosophila. The strong (17.4-fold) and highly specific induction of a single gene (CYP4D10) by the toxic isoquinoline alkaloids of a commonly utilized host-plant (saguaro cactus) provides the first indication that members of the CYP4 family in insects may play an important role in the maintenance of specific insect-host plant relationships. Strong barbiturate inducibility of CYP4D10 and two other D. mettleri P450 sequences of the CYP4 family was also observed, suggesting a pattern of xenobiotic responsiveness more similar to those of several vertebrate drug-metabolizing enzymes than to putative vertebrate CYP4 homologs.

Key words Cytochrome P450 Toxin resistance Multigene family Induction Drosophila 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • P. B. Danielson
    • 1
  • J. L. M. Foster
    • 2
  • M. M. McMahill
    • 1
  • M. K. Smith
    • 1
  • J. C. Fogleman
    • 1
  1. 1.Department of Biological Sciences, University of Denver, 2190 Iliff Avenue, Denver, CO 80208, USA Fax: +1-303-871-3471; e-mail: pdaniels@du.eduUS
  2. 2.Department of Biology, Metropolitan State College of Denver, Denver, CO 80217, USAUS

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