Molecular and General Genetics MGG

, Volume 258, Issue 5, pp 538–545

The stability of mRNA from the gsiB gene of Bacillus subtilis is dependent on the presence of a strong ribosome binding site

  • B. Jürgen
  • T. Schweder
  • M. Hecker
ORIGINAL PAPER

Abstract

In Bacillus subtilis IS58 starved of glucose or exposed to heat shock, ethanol or salt stress, the σB-dependent general stress protein GsiB is accumulated to a higher level than other general stress proteins. This high-level accumulation of GsiB can at least partially be attributed to the remarkably long half-life (∼20 min) of the gsiB mRNA. Analysis of different gsiB-lacZ fusions revealed that this stability is not determined by sequences at the 3 end of the transcript but rather by sequences upstream of the translational start codon. Site-directed mutagenesis established that a strong ribosome binding site was crucial for the increased stability of the gsiB mRNA. A comparison of the sequences upstream of the translational start codons of three general stress genes, gsiB, gspA and ctc, revealed a direct correlation between mRNA stability and the strength of their translational signals.

Key wordsBacillus subtilis General stress proteins gsiB mRNA stability Ribosome binding site 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • B. Jürgen
    • 1
  • T. Schweder
    • 1
  • M. Hecker
    • 1
  1. 1.Institut für Mikrobiologie und Molekularbiologie, Friedrich-Ludwig-Jahn-Straße 15, Ernst-Moritz-Arndt-Universität Greifswald, D-17487 Greifswald, Germany E-mail: hecker@microbio7.biologie.uni-greifswald.de Tel.: +49-3834-864200, Fax: +49-3834-864202DE

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