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Molecular and General Genetics MGG

, Volume 257, Issue 6, pp 686–692 | Cite as

Deletion of the Saccharomyces cerevisiae gene RAD30 encoding an Escherichia coli DinB homolog confers UV radiation sensitivity and altered mutability

  • A. A. Roush
  • M. Suarez
  • E. C. Friedberg
  • M. Radman
  • W. Siede
SHORT COMMUNICATION

Abstract

The dinB gene of Escherichia coli is an SOS-inducible gene of unknown function. Its mode of regulation and the amino acid sequence similarity of the predicted DinB protein to the UmuC protein of E. coli both suggest a role in cellular responses to DNA damage and probably in error-prone repair. Proteins with sequence similarity to DinB have been predicted from genes cloned from various prokaryotic and eukaryotic organisms, including Caenorhabditis elegans. Here we present the phenotypic characterization of a haploid Saccharomyces cerevisiae strain deleted for the ORF YDR419W, encoding a yeast DinB homolog. The deletion mutant is viable but is moderately sensitive to killing following exposure to ultraviolet (UV) radiation. Hence, we have named the gene RAD30. Steady-state levels of RAD30 transcripts are increased following UV irradiation. UV-induced locus-specific reversion of an ochre allele (arg4-17) is reduced in the rad30 deletion mutant. However, enhanced mutability was observed following treatment with the alkylating agent methylmethanesulfonate (MMS). Spontaneous mutability was also slightly increased. We conclude that RAD30 encodes an accessory function involved in DNA repair and mutagenesis. We speculate that the relatively weak phenotype and the opposite effects on mutability as a function of the type of DNA damage involved may derive from a functional redundancy of yeast proteins which facilitate replicative bypass of non-coding DNA lesions.

Key words DNA repair Mutagenesis Ultraviolet light Alkylating agents Yeast 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • A. A. Roush
    • 1
  • M. Suarez
    • 2
  • E. C. Friedberg
    • 3
  • M. Radman
    • 4
  • W. Siede
    • 2
  1. 1.Graduate Program in Biochemistry, Cellular and Developmental Biology, Emory University School of Medicine, Atlanta, GA 30322, USAUS
  2. 2.Department of Radiation Oncology and Winship Cancer Center, Emory University School of Medicine, Atlanta, GA 30322, USA. Tel.: +1-(404)-778-2170; Fax: 404-778-5016 E-mail: wsiede@emory.eduUS
  3. 3.Laboratory of Molecular Pathology, Department of Pathology, University of Texas Southwestern Medical Center, Dallas, TX 75235, USA US
  4. 4.CNRS, Institut Jacques Monod, Université Paris, Paris Cedex 05, FranceFR

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