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Molecular and General Genetics MGG

, Volume 256, Issue 4, pp 462–467 | Cite as

Antisense suppression of the putative ribosomal protein S3A gene disrupts ovarian development in Drosophila melanogaster

  • E. Reynaud
  • V. N. Bolshakov
  • V. Barajas
  • F. C. Kafatos
  • M. Zurita
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Abstract

The Drosophila melanogaster homologue of the Anopheles gambiae C3 cDNA has been isolated and characterized by sequence analysis. The encoded protein was localized by immunochemical and immunocytochemical methods. The Drosophila C3 protein is highly similar to homologues of disputed function, which have previously been identified in fungi, plants and animals. The protein is ubiquitous and localized in the cytoplasm. Cell fractionation followed by detection with a specific antibody preparation shows that the protein is associated with the 40S ribosomal subunit. The C3 gene is located in section 101F of chromosome 4. Antisense transgenic analysis shows that this gene is essential for oogenesis. The most prominent phenotype resulting from antisense depletion of C3 RNA is disappearance of the follicular cells of the ovary (where the concentration of C3 protein is normally high) and abnormalities of the associated germline derivatives, leading to failure of egg production.

Key wordsDrosophila Ribosomal protein Oogenesis Antisense Trangenesis 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • E. Reynaud
    • 1
  • V. N. Bolshakov
    • 2
  • V. Barajas
    • 1
  • F. C. Kafatos
    • 2
  • M. Zurita
    • 1
  1. 1.Departamento de Genética y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autonoma de México, APDO 510-3, 6221, Cuernavaca, Morelos, México Fax: 52-73-172388; e-mail: marioz@pbr322.ceingebi.unam.mx.
  2. 2.European Molecular Biology Laboratory, Meyerhofstrasse 1, D-69117 Heidelberg, GermanyDE

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