Molecular and General Genetics MGG

, Volume 256, Issue 3, pp 277–281

Genetic analysis of mutagenesis in aging Escherichia coli colonies

  • F. Taddei
  • J. A. Halliday
  • I. Matic
  • M. Radman
ORIGINAL PAPER

DOI: 10.1007/s004380050570

Cite this article as:
Taddei, F., Halliday, J., Matic, I. et al. Mol Gen Genet (1997) 256: 277. doi:10.1007/s004380050570

Abstract

Bacteria live in unstructured and structured environments, experiencing feast and famine lifestyles. Bacterial colonies can be viewed as model structured environments. SOS induction and mutagenesis have been observed in aging Escherichia coli colonies, in the absence of exogenous sources of DNA damage. This cAMP-dependent mutagenesis occurring in Resting Organisms in a Structured Environment (ROSE) is unaffected by a umuC mutation and therefore differs from both targeted UV mutagenesis and recA730 (SOS constitutive) untargeted mutagenesis. As a recB mutation has only a minor effect on ROSE mutagenesis it also differs from both adaptive reversion of the lacI33 allele and from iSDR (inducible Stable DNA Replication) mutagenesis. Besides its recA and lexA dependence, ROSE mutagenesis is also uvrB and polA dependent. These genetic requirements are reminiscent of the untargeted mutagenesis in λ phage observed when unirradiated λ infects UV-irradiated E. coli. These mutations, which are not observed in aging liquid cultures, accumulate linearly with the age of the colonies. ROSE mutagenesis might offer a good model for bacterial mutagenesis in structured environments such as biofilms and for mutagenesis of quiescent eukaryotic cells.

Key words Starvation Catabolite repression DNA repair SOS response Evolution 

Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • F. Taddei
    • 1
  • J. A. Halliday
    • 2
  • I. Matic
    • 1
  • M. Radman
    • 1
  1. 1.Laboratoire de Mutagenèse, Institut J. Monod, Université Paris 7, 2, Place Jussieu, F-75251 Paris Cedex 05, France Fax: +33-(1)-4427-5716; e-mail: taddei@ijm.jussieu.frFR
  2. 2.Dept. of Biological Sciences, Stanford University, Stanford, CA 94305, USAUS

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