Advertisement

Molecular and General Genetics MGG

, Volume 256, Issue 3, pp 239–251 | Cite as

Analysis of seven genes from the eryAI –eryK region of the erythromycin biosynthetic gene cluster in Saccharopolyspora erythraea

  • S. Gaisser
  • G. A. Böhm
  • J. Cortés
  • P. F. Leadlay
ORIGINAL PAPER

Abstract

The gene cluster (ery) governing the biosynthesis of the macrolide antibiotic erythromycin A by Saccharopolyspora erythraea contains, in addition to the eryA genes encoding the polyketide synthase, two regions containing genes for later steps in the pathway. The region 5′ of eryA, and lying between eryA and the gene eryK, which is known to encode the C-12 hydroxylase, has been sequenced and shown to contain seven additional open reading frames (ORFs 13–19). On the basis of sequence similarities, roles are proposed for several of these ORFs in the biosynthesis of the deoxysugar mycarose and the deoxyaminosugar desosamine. A chromosomal mutant carrying a deletion in ORF15 has been constructed and shown to accumulate 3-O-mycarosyl-erythronolide B, as expected for an eryC mutant. Similarly, a chromosomal mutant carrying a deletion in ORF16 has been constructed and shown to accumulate erythronolide B, as expected for an eryB mutant.

Key wordsSaccharopolyspora erythraea   Erythromycin biosynthetic genes   Desosamine   Mycarose    S-adenosylmethionine-methyltransferase 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • S. Gaisser
    • 1
  • G. A. Böhm
    • 1
  • J. Cortés
    • 1
  • P. F. Leadlay
    • 1
  1. 1.University of Cambridge, Department of Biochemistry, 80 Tennis␣Court Road, Cambridge CB2 IGA, UK Fax: +44-1223-333656; e-mail: pfl10@mole.bio.cam.ac.ukGB

Personalised recommendations