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Molecular and General Genetics MGG

, Volume 255, Issue 5, pp 487–494 | Cite as

Functional analysis of the recA promoter of Rhodobacter capsulatus

  • A. R. Fernàndez de Henestrosa
  • M. Labazi
  • M. Mar López
  • J. Barbé
ORIGINAL PAPER

Abstract

TTGT

ACTCATACCATGAGA

ACAA

, which is centered on position-8 with respect to the transcriptional starting site. PCR mutagenesis of both halves of this palindrome indicates that the TTGT and ACAA motifs are necessary both for normal DNA-protein complex formation in vitro and for full DNA damage-mediated induction of recA. Nevertheless, the basal level of recA expression is not increased when both halves of the TTGTN15ACAA sequence are mutagenized. These data suggest that the R. capsulatus recA gene may be regulated by a positive transcription factor which binds to this palindrome.

Key wordsRhodobacter capsulatus recA promoter DNA-binding protein Positive regulation 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • A. R. Fernàndez de Henestrosa
    • 1
  • M. Labazi
    • 1
  • M. Mar López
    • 1
  • J. Barbé
    • 1
  1. 1.Department of Genetics and Microbiology, Universitat Autónoma of Barcelona, Bellaterra, E-08193 Barcelona, SpainES

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